IDCASES - 2020 - Management of Dengue Hemorrhagic Fever in a Secondary Level Hospital in Bangladesh: A Case Report. Introduction Dengue fever (DF) is a mosquito borne (family Flaviviridae, genus Flavivirus) disease of humans considered as a temporal worldwide public health concern .
The disease is now hyperendemic both in tropical and subtropical regions circulating all four serotypes (DENV-1 to DENV-4) . Globally, almost 4 billion people from at least 128 countries are under the risk  and estimated 50 million dengue infections are occurring each year . The World Health Organization declared South Asia as an endemic area for the disease due to favorable dissemination environment of Aedes aegypti, the main dengue vector .
The clinical spectrum of dengue disease manifestations ranges from asymptomatic to symptomatic forms like dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) . Case report A 42-year-old patient came to a secondary level hospital with complaints of diffuse abdominal pain (more in central region) continually for 3 days. Fig. 1. Table 1. Discussion Conclusion. VIROLOGY JOURNAL 15/08/19 Evaluation of novel rapid detection kits for dengue virus NS1 antigen in Dhaka, Bangladesh, in 2017. Selection of MAbs against DENV-NS1 for RDT To develop an NS1 antigen detection RDT, we obtained 12 MAbs against DENV-NS1 from Bio Matrix Research, Inc.
To increase testing throughput, we used a dipstick format, which permitted completion of each test in approximately 15 min (depending on humidity conditions). We conjugated the MAbs with AuNPs, adsorbed the MAbs onto nitrocellulose membranes, and tested all combination of MAbs by placing dipsticks in microcentrifuge tubes or the wells of a 96-well plate containing 60 μL of extraction buffer and 30 μL of sample specimen per tube or well. First, we tested recombinant NS1 proteins of all four serotypes of DENV and selected 12 combinations that showed signal/background ratios greater than 2.0. The high percentage of amino acid homology and identity among flavivirus NS1 proteins has been reported previously .
Competitive ELISA using synthetic peptides. PLOS 12/11/18 Geostatistical mapping of the seasonal spread of under-reported dengue cases in Bangladesh. Abstract Geographical mapping of dengue in resource-limited settings is crucial for targeting control interventions but is challenging due to the problem of zero-inflation because many cases are not reported.
We developed a negative binomial generalised linear mixed effect model accounting for zero-inflation, spatial, and temporal random effects to investigate the spatial variation in monthly dengue cases in Bangladesh. The model was fitted to the district-level (64 districts) monthly reported dengue cases aggregated over the period 2000 to 2009 and Bayesian inference was performed using the integrated nested Laplace approximation. We found that mean monthly temperature and its interaction with mean monthly diurnal temperature range, lagged by two months were significantly associated with dengue incidence.
Mean monthly rainfall at two months lag was positively associated with dengue incidence. Author summary Editor: Christopher M. Copyright: © 2018 Sharmin et al. Introduction Fig 1. Data. PLOS 21/06/18 Risk factors for the presence of dengue vector mosquitoes, and determinants of their prevalence and larval site selection in Dhaka, Bangladesh. Abstract Dengue viruses are responsible for over 100 million infections a year worldwide and are a public health concern in Bangladesh.
Although risk of transmission is high, data on vector population characteristics are scanty in Bangladesh; therefore, a comprehensive prediction of the patterns of local virus transmission is not possible. Recognizing these gaps, multi-year entomological surveys were carried out in Dhaka, where the disease is most frequently reported. The specific objectives of the present study are threefold: i) to determine the risk factors for the presence of Aedes mosquitoes; ii) to identify the types of most productive and key containers; and iii) to estimate the effects of climatic factors on Aedes abundance in the city of Dhaka, Bangladesh. Entomological surveys were conducted in 12 out of 90 wards in Dhaka. Editor: Olle Terenius, Swedish University of Agricultural Sciences, SWEDEN Received: January 10, 2018; Accepted: June 7, 2018; Published: June 21, 2018. Virusdisease. 2018 Sep;29(3):303-307. Circulating dengue virus serotypes in Bangladesh from 2013 to 2016.
INDIAN JOURNAL OF MEDICAL RESEARCH - 2012 - Advances in developing a climate based dengue outbreak models in Dhaka, Bangladesh: Challenges & opportunities. PLOS 23/03/17 Dengue seroprevalence, seroconversion and risk factors in Dhaka, Bangladesh. Abstract Background Dengue virus (DENV) activity has been reported in Dhaka, Bangladesh since the early 1960s with the greatest burden of dengue fever and dengue hemorrhagic fever cases observed in 2000.
Since this time, the intensity of dengue activity has varied from year to year, and its determining factors remained relatively unknown. In light of such gaps in knowledge, the main objectives of this study were to determine the magnitude of seroprevalence and seroconversion among the surveyed population, and establish the individual/household level risk factors for the presence of DENV antibodies among all age groups of target populations in the city of Dhaka.
Methodology/Principal findings Considering the lack of fine scale investigations on the factors driving dengue activity in Bangladesh, a prospective cohort study involving serological surveys was undertaken with participant interviews and blood donation across the city of Dhaka in 2012. Conclusions/Significance Author summary Methods.