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Champignons transgéniques

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Appl Microbiol Biotechnol. 2020 Feb CRISPR-Cas9 Assisted Functional Gene Editing in the Mushroom Ganoderma Lucidum. Arazoe T, Miyoshi K, Yamato T, Ogawa T, Ohsato S, Arie T, Kuwata S (2015) Tailor-made CRISPR/Cas system for highly efficient targeted gene replacement in the rice blast fungus.

Appl Microbiol Biotechnol. 2020 Feb CRISPR-Cas9 Assisted Functional Gene Editing in the Mushroom Ganoderma Lucidum

Biotechnol Bioeng 112(12):2543–2549. Article PubMed Google Scholar Brow DA, Guthrie C (1988) Spliceosomal RNA U6 is remarkably conserved from yeast to mammals. Nature 334(6179):213–218. RFI 08/06/19 Paludisme: un champignon OGM anti-moustique. POURQUOI DOCTEUR 02/06/19 Paludisme : un champignon génétiquement modifié capable de tuer les moustiques. La lutte contre les moustiques est un enjeu de santé majeur dans le monde.

POURQUOI DOCTEUR 02/06/19 Paludisme : un champignon génétiquement modifié capable de tuer les moustiques

Ces insectes sont vecteurs de plusieurs maladies potentiellement mortelles, comme le paludisme. Des chercheurs burkinabés et américains sont parvenus à trouver une méthode pour tuer les moustiques porteurs de ce virus, grâce à un champignon génétiquement modifié. Une toxine issue du venin d'une araignée Le champignon, appelé Metarhizium pingshaense, infecte naturellement les insectes dans l’environnement. Il est capable de les tuer lentement, mais les chercheurs ont voulu le rendre plus efficace. LE POINT 31/05/19 Un champignon OGM pourrait éradiquer le paludisme. PLOS 07/09/18 Transgenic Metarhizium pingshaense synergistically ameliorates pyrethroid-resistance in wild-caught, malaria-vector mosquitoes. Abstract Transgenic Metarhizium pingshaense expressing the spider neurotoxin Hybrid (Met-Hybrid) kill mosquitoes faster and at lower spore doses than wild-type strains.

PLOS 07/09/18 Transgenic Metarhizium pingshaense synergistically ameliorates pyrethroid-resistance in wild-caught, malaria-vector mosquitoes

In this study, we demonstrate that this approach dovetails with the cornerstone of current malaria control: pyrethroid-insecticides, which are the cornerstone of current malaria control. We used World Health Organization (WHO) tubes, to compare the impact on insecticide resistance of Met-Hybrid with red fluorescent M. pingshaense (Met-RFP), used as a proxy for the wild-type fungus. Insecticides killed less than 20% of Anopheles coluzzii and Anopheles gambiae s.s. mosquitoes collected in a malaria endemic region of Burkina Faso where pyrethroid use is common.

Seven days post-infection, mortality for insecticide-sensitive and resistant mosquitoes averaged 94% with Met-Hybrid and 64% with Met-RFP, with LT80 values of 5.32±0.199 days and 7.76±0.183 days, respectively. Background. WIPO - 2007 - immunisation par champignons transgeniques. NATURE 14/04/16 Gene-edited CRISPR mushroom escapes US regulation - A fungus engineered with the CRISPR–Cas9 technique can be cultivated and sold without further oversight. Jose A.

NATURE 14/04/16 Gene-edited CRISPR mushroom escapes US regulation - A fungus engineered with the CRISPR–Cas9 technique can be cultivated and sold without further oversight.

GENE EDITING 18/10/16 Questions asked on CRISPR-edited mushrooms bypassing GM regulation. In an April 2016 commentary in Nature[1], a genetically modified mushroom, created by Yinong Yang, a scientist at Pennsylvania State University, was reported in which had bypassed regulation from the US Department of Agriculture.

GENE EDITING 18/10/16 Questions asked on CRISPR-edited mushrooms bypassing GM regulation

The modification deletes part of a gene in the polyphenol oxidase (PPO) family, which is responsible for the mushrooms going brown. The edited mushrooms can then be left on the supermarket shelf longer without going off. This loophole was due to the type of gene editing being performed, which used the CRISPR/Cas9 approach to knock out the gene. Because no foreign DNA was detected at the editing site, it was considered not applicable for regulation. Now, a new correspondence in Nature Biotechnology[2] from Jungeun Kim & Jin-Soo Kim from the Seoul National University, has questioned this decision.

In terms of GM food created by CRISPR/Cas9 gene editing, we are just at the very start of a very big wave. FOOD SAFETY MAGAZINE 21/04/16 Genetically Modified Mushrooms One Step Closer to Store Shelves. News | April 21, 2016 By Staff The U.S.

FOOD SAFETY MAGAZINE 21/04/16 Genetically Modified Mushrooms One Step Closer to Store Shelves

Department of Agriculture (USDA) has decided that it will not regulate the cultivation or sale of a genetically modified mushroom. With the help of the CRISPR gene-editing tool and a plant pathologist at Pennsylvania State University, this mushroom will be the first of its kind utilizing that tool to escape federal oversight. The specific mushroom fungus undergoing modification is Agaricus bisporus, a variety of popular white button mushrooms. Gene-editing reduces browning--a common and frustrating characteristic for those who consume mushrooms. This mushroom is not the first genetically modified food item that the USDA has granted immunity from regulation. Despite getting past the USDA, the mushroom may still need to be approved by the U.S. IOBLOG 19/04/16 CRISPR GM mushrooms do not require USDA approval. A mushroom created using CRISPR genetic modification The US Department of Agriculture (USDA) said that it is not necessary to regulate the cultivation and sale of genetically modified mushrooms because they do not contain any foreign DNA.

IOBLOG 19/04/16 CRISPR GM mushrooms do not require USDA approval

That decision is unprecedented for genetically modified organisms using CRISPR, a relatively new genetic modification technique that is best known for its applications in the human embryos than in vegetables. Unlike older methods used to create GMOs, CRISPR does not insert the DNA of viruses and bacteria in target organisms to make the proper changes, and for that reason, the GM mushrooms do not fall within the rules of USDA.

The USDA SAYS THAT THE LACK OF FOREIGN DNA PRESENTS NO THREAT TO OTHER PLANTS. AGROMEDIA 30/06/14 Un édulcorant produit à partir de champignons OGM. ENGADGET 16/04/16 Genetically modified mushrooms cleared by the USDA. INFOGM 26/10/16 États-Unis - Le champignon OGM (Crispr) doit-il être évalué ? En avril 2016, le gouvernement étasunien déclare qu’un champignon de Paris génétiquement modifié par Crispr/Cas9 ne sera pas soumis à la loi sur les produits issus d’ingénierie génétique (nos OGM européens).

INFOGM 26/10/16 États-Unis - Le champignon OGM (Crispr) doit-il être évalué ?

Six mois plus tard, des scientifiques coréens contestent la base scientifique ayant permis cette décision. Une controverse qui ne remettra pourtant pas en cause la décision du gouvernement. Récemment le champignon Agaricus bisporus a été génétiquement modifié pour ne pas brunir au contact de l’air. Un nom latin peu connu sous lequel se cache en fait le « fameux » champignon de Paris. Yinong Yang, de l’Université d’état de Pennsylvannie, a réussi à supprimer certaines paires de bases de la séquence génétique codant pour l’enzyme polyphénol oxydase, en utilisant Crispr/Cas9, une des nouvelles techniques de modification du vivant.

Aspergillus niger transgénique.