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Actualités anglophones concernant la paratransgenese

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INTECH - AVRIL 2017 - Biological Control of Pest and Vector Insects Au sommaire notamment : Developing the Arsenal Against Pest and Vector Dipterans: Inputs of Transgenic and Paratransgenic Biotechnologies. David Bethune VIA YOUTUBE 15/09/16 Miami Used Naled Spraying to Hide GMO Mosquito Agenda. David Bethune VIA YOUTUBE 10/09/16 Citizens Defeat GMO Mosquito Resolution (Part 1) Al Jazeera English via YOUTUBE 04/12/16 Can genetically-modified mosquitoes help eradicate malaria? - TechKnow. FDA 05/08/16 Environmental Assessment for Investigational Use of Aedes aegypti OX513A. ECOLOGY AND EVOLUTION 12/09/13 Release of genetically engineered insects: a framework to identify potential ecological effects. Abstract Genetically engineered (GE) insects have the potential to radically change pest management worldwide.

ECOLOGY AND EVOLUTION 12/09/13 Release of genetically engineered insects: a framework to identify potential ecological effects

With recent approvals of GE insect releases, there is a need for a synthesized framework to evaluate their potential ecological and evolutionary effects. The effects may occur in two phases: a transitory phase when the focal population changes in density, and a steady state phase when it reaches a new, constant density. We review potential effects of a rapid change in insect density related to population outbreaks, biological control, invasive species, and other GE organisms to identify a comprehensive list of potential ecological and evolutionary effects of GE insect releases.

We apply this framework to the Anopheles gambiae mosquito – a malaria vector being engineered to suppress the wild mosquito population – to identify effects that may occur during the transitory and steady state phases after release. Introduction Conceptual framework Box 1. Transitory Phase Gene flow. Bull World Health Organ 2016;94:766–771 Use of transgenic Aedes aegypti in Brazil: risk perception and assessment.

PLOS 06/10/16 What Is Stopping the Use of Genetically Modified Insects for Disease Control? Citation: Panjwani A, Wilson A (2016) What Is Stopping the Use of Genetically Modified Insects for Disease Control?

PLOS 06/10/16 What Is Stopping the Use of Genetically Modified Insects for Disease Control?

PLoS Pathog 12(10): e1005830. doi:10.1371/journal.ppat.1005830 Editor: Carolyn B. Coyne, University of Pittsburgh, UNITED STATES Published: October 6, 2016 Copyright: © 2016 Panjwani, Wilson. Funding: AP is supported by the United Kingdom’s Medical Research Council [grant number MR/K020811/1]. Competing interests: I have read the journal's policy and have the following conflicts: since March 2015, AW has received funding by BBSRC to develop insect population models which could be used to help improve release strategies for GM insect vectors (grant reference BB/M017567/1).

Insect-borne pathogens impose a substantial burden on health, the environment, and agricultural production, and rapid outbreaks of such pathogens are becoming more common [e.g., 1,2]. Regional differences also reflect public opinion. BMJ 27/05/16 Can genetically modified mosquitoes stop Zika? No, but genetically modified bacteria can. REUTERS 05/08/16 GM mosquito trial will not significantly impact environment: FDA. THE SCIENTIST 05/08/16 FDA: GM Mosquitoes Safe for Environment. PLOS 02/07/15 Suppression of a Field Population of Aedes aegypti in Brazil by Sustained Release of Transgenic Male Mosquitoes.

Abstract The increasing burden of dengue, and the relative failure of traditional vector control programs highlight the need to develop new control methods.

PLOS 02/07/15 Suppression of a Field Population of Aedes aegypti in Brazil by Sustained Release of Transgenic Male Mosquitoes

SIT using self-limiting genetic technology is one such promising method. A self-limiting strain of Aedes aegypti, OX513A, has already reached the stage of field evaluation. Sustained releases of OX513A Ae. aegypti males led to 80% suppression of a target wild Ae. aegypti population in the Cayman Islands in 2010. MEM INST OSWALDO CRUZ, RIO DE JANEIRO, 109(5) August 2014 Metagenomics, paratransgenesis and the Anopheles microbiome:a portrait of the geographical distribution of the anopheline microbiota based on a meta-analysis of reported taxa.

CDC EID – FEV 2015 - Awareness and Support of Release of Genetically Modified “Sterile” Mosquitoes, Key West, Florida, USA. Author affiliations: University of Arizona, Tucson, Arizona, USA (K.C.

CDC EID – FEV 2015 - Awareness and Support of Release of Genetically Modified “Sterile” Mosquitoes, Key West, Florida, USA.

Ernst, S. Haenchen, K. Walker); National Center of Atmospheric Research, Boulder, Colorado, USA (K. Dickinson, M.H. Hayden, A.J. PLOS 13/03/14 A Regulatory Structure for Working with Genetically Modified Mosquitoes: Lessons from Mexico. Citation: Ramsey JM, Bond JG, Macotela ME, Facchinelli L, Valerio L, Brown DM, et al. (2014) A Regulatory Structure for Working with Genetically Modified Mosquitoes: Lessons from Mexico.

PLOS 13/03/14 A Regulatory Structure for Working with Genetically Modified Mosquitoes: Lessons from Mexico

PLoS Negl Trop Dis 8(3): e2623. doi:10.1371/journal.pntd.0002623 Editor: Jesus G. Valenzuela, National Institute of Allergy and Infectious Diseases, United States of America Published: March 13, 2014 Copyright: © 2014 Ramsey et al. Funding: Research and Policy for Infectious Disease Dynamics program of the Science and Technology Directory, Department of Homeland Security; Fogarty International Center, National Institutes of Health; Pasteur Institute – Cenci Bolognetti Foundation; Foundation for the National Institutes of Health through the Grand Challenges in Global Health (GCGH) initiative.

PAHO - AOUT 2014 - Technical note on transgenic mosquitoes engineered for Aedes aegypti control. J Med Entomol. 2012 Nov;49(6):1177-88. Mathematical models as aids for design and development of experiments: the case of transgenic mosquitoes. BMC 23/06/15 A delivery system for field application of paratransgenic control. Bacterial culture P. agglomerans E325 culture was maintained on Luria Bertani (LB) agar at 30 °C or in LB broth at 30 °C at 180 rpm.

BMC 23/06/15 A delivery system for field application of paratransgenic control

Preparation P. agglomerans E325 competent cells Bacterial cells were grown in 100 ml of LB medium at 30 °C with shaking at 175–180 rpm. When OD600 was reached 0.4-0.6 the cells were harvested by centrifugation at 4000 rpm for 10 min at 4 °C. These cells were resuspended in 40 ml of ice cold Inoue solution and harvested by centrifugation at 4000 rpm for 10 min at 4 °C. OMS 26/06/14 A new framework for evaluating genetically modified mosquitoes. Insects 2015, 6(1), 236-278 Fighting Arbovirus Transmission: Natural and Engineered Control of Vector Competence in Aedes Mosquitoes. CDC EID – FEV 2015 - Awareness and Support of Release of Genetically Modified “Sterile” Mosquitoes, Key West, Florida, USA. ISAUDE 01/07/13 Largest project in the world loose 430 million modified Aedes aegypti in BahiaWill be released into the environm.

A new stage design Transgenic Aedes (PAT), which frees males genetically modified to combat wild insect, that transmits dengue, was started in the city of Bahia Jacobina.

ISAUDE 01/07/13 Largest project in the world loose 430 million modified Aedes aegypti in BahiaWill be released into the environm

Will be released approximately 4 million mosquitoes a week for the next three years to combat dengue. Result of an agreement between the Institute of Biomedical Sciences (ICB) and the USP biofactory Moscamed Brazil, PAT will release in this new stage, more than 430 million mosquitoes. "This is a pioneering project in the world.

Nowhere was made a release of transgenic mosquitoes that size, the researcher afima ICB, Margareth Lara Capurro, coordinator of PAT. Lineage insect used was developed by British scientists Company Oxitec, partner in the project, and is prouzida Unit Production of Transgenic Aedes Moscamed in Juazeiro, today with a weekly production of approximately 1 million males. The release of male mosquito Aedes aegypti is a genetically modified disease efficient technique. The first step <b></ b> OBC/wprs Bulletin Vol. 73, 2012 Defining environmental risk assessment criteria for GM insects to be placed on the EU market.

HOUSES OF PARLIAMENT - JUNE 2010 - Genetically Modified Insects. Parasites & Vectors 2011, 4:82 (19 May 2011) The paratransgenic sand fly: A platform for control of Leishmania transmission. THE NEW YORKER 09/07/12 The Mosquito Solution - Can genetic modification eliminate a deadly tropical disease? Rev. Inst. Med. trop. S. Paulo vol.54 no.5 São Paulo Sept./Oct. 2012 Genetic Control of Mosquitoes: population suppression strat. Genetic Control of Mosquitoes: population suppression strategies Controle genético de mosquitos: estratégias de supressão de populações André Barretto Bruno Wilke; Mauro Toledo Marrelli Department of Epidemiology, Faculdade de Saúde Pública, University of São Paulo, São Paulo, SP, Brazil Correspondence to Over the last two decades, morbidity and mortality from malaria and dengue fever among other pathogens are an increasing Public Health problem.

Rev. Inst. Med. trop. S. Paulo vol.54 no.5 São Paulo Sept./Oct. 2012 Genetic Control of Mosquitoes: population suppression strat

Keywords: Mosquito; Genetic control; SIT; RIDL; Field tests. Ao longo das duas últimas décadas, morbidade e mortalidade da malária e dengue e outros patógenos tem se tornado cada vez mais um problema de Saúde Pública. Arthropods act as vectors for many human agents that cause several diseases30. The increase in the geographic distribution of vectors is accompanied by the emergence of viruses and diseases in new areas. The Sterile Insect Technique (SIT) is a species-specific and environmentally benign method for insect population control24,32. Peer Reviewed Journal 03/01/13 Transgenic sexing system for Ceratitis capitata (Diptera: Tephritidae) based on female-specific e. Int J Biol Sci 2011; 7:1334-1344 Paratransgenic Control of Vector Borne Diseases 1. Institute of Dentistry, Queen Mary, Univers. INTECH - FEV 2012 - Transgenesis, Paratransgenesis and Transmission Blocking Vaccines to Prevent Insect-Borne Diseases.

PLOS 21/04/11 Engineered Resistance to Plasmodium falciparum Development in Transgenic Anopheles stephensi. Abstract Transposon-mediated transformation was used to produce Anopheles stephensi that express single-chain antibodies (scFvs) designed to target the human malaria parasite, Plasmodium falciparum.

PLOS 21/04/11 Engineered Resistance to Plasmodium falciparum Development in Transgenic Anopheles stephensi

The scFvs, m1C3, m4B7, and m2A10, are derived from mouse monoclonal antibodies that inhibit either ookinete invasion of the midgut or sporozoite invasion of salivary glands. The scFvs that target the parasite surface, m4B7 and m2A10, were fused to an Anopheles gambiae antimicrobial peptide, Cecropin A. Previously-characterized Anopheles cis-acting DNA regulatory elements were included in the transgenes to coordinate scFv production with parasite development. Gene amplification and immunoblot analyses showed promoter-specific increases in transgene expression in blood-fed females.

Author Summary Malaria eradication will require vector-control strategies that are both self-sustaining and not affected by migration of infected humans and mosquitoes. Figures Editor: Nora J. Introduction.