VIRUSES 30/10/20 The Antiviral Small-Interfering RNA Pathway Induces Zika Virus Resistance in Transgenic Aedes aegypti. The resurgence of arbovirus outbreaks across the globe, including the recent Zika virus (ZIKV) epidemic in 2015–2016, emphasizes the need for innovative vector control methods.
In this study, we investigated ZIKV susceptibility to transgenic Aedes aegypti engineered to target the virus by means of the antiviral small-interfering RNA (siRNA) pathway. The robustness of antiviral effector expression in transgenic mosquitoes is strongly influenced by the genomic insertion locus and transgene copy number; we therefore used CRISPR/Cas9 to re-target a previously characterized locus (Chr2:321382225) and engineered mosquitoes expressing an inverted repeat (IR) dsRNA against the NS3/4A region of the ZIKV genome. Small RNA analysis revealed that the IR effector triggered the mosquito’s siRNA antiviral pathway in bloodfed females. Nearly complete (90%) inhibition of ZIKV replication was found in vivo in both midguts and carcasses at 7 or 14 days post-infection (dpi). ►▼ Show Figures Figure 1. SCIENTIFIC REPORTS 10/09/19 Transgenic Aedes aegypti Mosquitoes Transfer Genes into a Natural Population.
Release and rearing sites Jacobina, in the state of Bahia, Brazil, is a moderately sized city of ~75,000 inhabitants located at coordinates 11°10′51″S, 40°31′04″W (Fig. 1).
Jacobina is surrounded for several kilometers in all directions by caatinga, a dry ecological biome in which Ae. aegypti cannot breed, making Jacobina an island for this mosquito. PLOS 17/11/11 Transcriptome Analysis of Aedes aegypti Transgenic Mosquitoes with Altered Immunity. Citation: Zou Z, Souza-Neto J, Xi Z, Kokoza V, Shin SW, Dimopoulos G, et al. (2011) Transcriptome Analysis of Aedes aegypti Transgenic Mosquitoes with Altered Immunity.
PLoS Pathog 7(11): e1002394. Editor: James W. NATURE SCIENTIFIC REPORTS 10/09/19 Transgenic Aedes aegypti Mosquitoes Transfer Genes into a Natural Population. ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA 07/07/17 Effect of interruption of over-ﬂooding releases of transgenic mosquitoes over wild population of Aedes aegypti: two case studies in Brazil. J Med Entomol. 2012 Nov;49(6):1177-88. Mathematical models as aids for design and development of experiments: the case of transgenic mosquitoes.
SCIENCEMAG 17/09/19 Study on DNA spread by genetically modified mosquitoes prompts backlash. For 10 years, the company Oxitec has been testing whether genetically modified (GM) mosquitoes can suppress populations of their natural brethren, which carry devastating viruses such as Zika and dengue.
Its strategy: Deploy (nonbiting) male Aedes aegypti mosquitoes bearing a gene that should doom most of their offspring before adulthood. Now, a team of independent researchers analyzing an early trial of Oxitec’s technology is raising alarm—and drawing fire from the firm—with a report that some offspring of the GM mosquitoes survived and produced offspring that also made it to sexual maturity.
As a result, local mosquitoes inherited pieces of the genomes of the GM mosquitoes, the team revealed last week in Scientific Reports. There’s no evidence that these hybrids endanger humans more than the wild mosquitoes or that they’ll render Oxitec’s strategy ineffective, both the paper’s authors and the company agree. FRESH FRUIT PORTAL 29/01/20 First open-field trial of GM moth 'could herald new era of crop protection'
A new study reports a successful, first-ever open-field release of a self-limiting, genetically engineered diamondback moth.
Researchers believe that the results of the study pave the way for an effective and sustainable approach to pest control. The diamondback moth, also known as Plutella xylostella, is highly damaging to brassica crops such as cabbage, broccoli, cauliflower and canola. This new strain of diamondback moth, developed by U.K. -based Oxitec, is modified to control pest diamondback moth in a targeted manner. The study showed the engineered strain had similar field behaviors to unmodified diamondback moths, with results offering promise for future protection of farmers’ brassica crops. The Cornell study was led by Professor Anthony Shelton in the Department of Entomology at Cornell University’s AgriTech in New York and has been published in Frontiers in Bioengineering and Biotechnology. Male diamondback moths as a crop protection solution. Front. Bioeng. Biotechnol., 29 January 2020 First Field Release of a Genetically Engineered, Self-Limiting Agricultural Pest Insect: Evaluating Its Potential for Future Crop Protection.
Introduction Arthropod pests cause an estimated >$470 billion in lost agricultural crops worldwide (Culliney, 2014).
The main tool for controlling such pests is the use of insecticides, the global annual market value of which is projected to reach $16.44 billion by 2019 (Statistica, 2019). BMC MICROBIOLOGY 23/11/18 Towards improving tsetse fly paratransgenesis: stable colonization of Glossina morsitans morsitans with genetically modified Sodalis. As vector-borne diseases continue to present significant threats to human, animal and plant health, there is a perpetual need to improve existing and develop new control strategies. Vector control has always been and still remains a key component of any integrated program to control vector-borne diseases.
In the light of increasing insecticide resistance combined with the growing awareness of the negative environmental and ecological consequences caused by the widespread use of insecticides, genetic control of the insect vector provides a valuable addition to the armory of tools already available. Tsetse flies (Glossina sp.) are medically and agriculturally important vectors that transmit Trypanosoma spp. parasites responsible for human sleeping sickness and animal African trypanosomiasis (AAT). UNICAMP_BR - 2019 - Dissertation en ligne : Dengue's fluctuation in a middle city, with genetically modified mosquitoes liberation : a longitudinal study. TRENDS IN PARASITOLOGY - JUNE 2018 - Transgenic Mosquitoes – Fact or Fiction? THECONVERSATION 20/08/18 Genetically modified mosquitoes may be best weapon for curbing disease transmission. Mosquitoes are some of the most deadly creatures on the planet.
They carry viruses, bacteria and parasites, which they transmit through bites, infecting some 700 million people and killing more than 1 million each year. With international travel, migration and climate change, these infections are no longer confined to tropical and subtropical developing countries. Pathogens such as West Nile virus and Zika virus have caused significant outbreaks in the United States and its territories that are likely to continue, with new invasive pathogens being discovered all the time.
Currently, control of these diseases is mostly limited to broad-spectrum insecticide sprays, which can harm both humans and non-target animals and insects. What if there was a way to control these devastating diseases without the environmental problems of widespread insecticide use? I have been working on GM mosquitoes, both as a lab tool and to combat disease, for over 20 years. A bacterium that fights disease. PNAS 26/02/19 Engineered resistance to Zika virus in transgenic Aedes aegypti expressing a polycistronic cluster of synthetic small RNAs.
PARASITES & VECTORS 21/12/18 Molecular tools and genetic markers for the generation of transgenic sexing strains in Anopheline mosquitoes. NATURE 08/10/18 Blood meal-induced inhibition of vector-borne disease by transgenic microbiota. IFLSCIENCE 17/04/19 How Genetically Edited Doublesex Mosquitoes Could Save Millions Of Lives. Syndicated from the blog of Bill Gates.
Bill Gates is a financial sponsor of IFLScience. It’s Mosquito Week again on the Gates Notes. This year I’m exploring some of the science behind malaria and other mosquito-borne diseases. You can read below about how gene editing could play a key role in eradicating malaria. I’ve also written about amazing advances in tracking the disease and how the parasite is a deadly shapeshifter. AFRICAN CENTRE FOR BIODIVERSITY 02/05/19 STOP RISKY GM MOSQUITO RELEASES – WE HAVE THE RIGHT TO SAY NO. We, the undersigned civil society organisations in Africa, hereby call upon the Bill and Melinda Gates Foundation and the Target Malaria project to stop the intended release of 10 000 genetically modified (GM) “male sterile” mosquitoes in Burkina Faso, as the release poses unacceptable risks to human beings and the environment.
We note with grave concern that African citizens are being exposed to life threatening health risks, through questionable technology involving the release of GM mosquitoes. We are even more alarmed to learn that, by Target Malaria’s own admission, there are no advantages expected from the initial proposed GM mosquito release, as it is intended only for training purposes and not expected to deliver any benefits for malaria control in Burkina Faso. There is absolutely no justification for releasing these GM mosquitoes in Burkina Faso or elsewhere on the continent. We note with concern that the Target Malaria project is shrouded in secrecy. CORNELL ALLIANCE FOR SCIENCE 05/09/17 First US field trials under way for self-limiting "GMO" insect. Scientists at Cornell University have confirmed that they have begun releasing genetically engineered diamondback moths into an isolated field on a research farm in upstate New York.
The focus of the project is to investigate the potential for biotechnology to reduce insecticide use in vegetable farming. Diamondback moths, a serious pest of cabbage and other brassica vegetable and field crops, are an invasive pest in the United States. Vector-Borne and Zoonotic Diseases 01/01/18 Studies of Transgenic Mosquitoes in Disease-Endemic Countries: Preparation of Containment Facilities. PLOS 26/07/18 Genetically engineered mosquitoes, Zika and other arboviruses, community engagement, costs, and patents: Ethical issues.
Citation: Meghani Z, Boëte C (2018) Genetically engineered mosquitoes, Zika and other arboviruses, community engagement, costs, and patents: Ethical issues. PLoS Negl Trop Dis 12(7): e0006501. Editor: Paulo Pimenta, Fundaçao Oswaldo Cruz, BRAZIL Published: July 26, 2018 Copyright: © 2018 Meghani, Boëte. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: The authors received no specific funding for this work. Competing interests: The authors have declared that no competing interests exist. Introduction. VECTOR-BORNE AND ZOONOTIC DISEASES 01/01/18 Containment Studies of Transgenic Mosquitoes in Disease Endemic Countries: The Broad Concept of Facilities Readiness.
Labiotech.eu VIA YOUTUBE 21/06/17 Synbio: Genetically modified mosquitos against diseases - Hadyn Parry, Oxitec's CEO. Social Epistemology - 2018 - Matters of Dwelling: Releasing the Genetically Engineered Aedes Aegypti Mosquito in Key West. VECTOR-BORNE AND ZOONOTIC DISEASES - JANV 2018 - Studies of Transgenic Mosquitoes in Disease-Endemic Countries: Preparation of Containment Facilities. About This Journal... To cite this article:Quinlan M. Megan, Mutunga James Mutuku, Diabaté Abdoulaye, Namountougou Moussa, Coulibaly Mamadou B., Sylla Lakamy, Kayondo Jonathan, Balyesima Victor, Clark Lorna, Benedict Mark Q., and Raymond Peter. Vector-Borne and Zoonotic Diseases.
January 2018, 18(1): 21-30. VECTOR-BORNE AND ZOONOTIC DISEASES - JANV 2018 - Maintaining Quality of Candidate Strains of Transgenic Mosquitoes for Studies in Containment Facilities in Disease Endemic Countries s9" About This Journal... To cite this article:Mumford John D., Leach Adrian W., Benedict Mark Q., Facchinelli Luca, and Quinlan M. Megan. Vector-Borne and Zoonotic Diseases. January 2018, 18(1): 31-38. EUREKALERT 28/09/17 Disease resistance successfully spread from modified to wild mosquitoes. WHAT: Using genetically modified (GM) mosquitoes to reduce or prevent the spread of infectious diseases is a new but rapidly expanding field of investigation.
Among the challenges researchers face is ensuring that GM mosquitoes can compete and mate with their wild counterparts so the desired modification is preserved and spread in the wild population. Investigators at Johns Hopkins University have engineered GM mosquitoes to have an altered microbiota that suppresses human malaria-causing parasites. These GM mosquitos preferred to mate with wild mosquitoes and passed along the desired protection to many generations of offspring.
NIH 28/09/17 Disease resistance successfully spread from modified to wild mosquitoes NIAID-funded group assesses mating of genetically modified species. INTECH - AVRIL 2017 - Biological Control of Pest and Vector Insects Au sommaire notamment : Developing the Arsenal Against Pest and Vector Dipterans: Inputs of Transgenic and Paratransgenic Biotechnologies.
David Bethune VIA YOUTUBE 15/09/16 Miami Used Naled Spraying to Hide GMO Mosquito Agenda. David Bethune VIA YOUTUBE 10/09/16 Citizens Defeat GMO Mosquito Resolution (Part 1) Al Jazeera English via YOUTUBE 04/12/16 Can genetically-modified mosquitoes help eradicate malaria? - TechKnow. FDA 05/08/16 Environmental Assessment for Investigational Use of Aedes aegypti OX513A. ECOLOGY AND EVOLUTION 12/09/13 Release of genetically engineered insects: a framework to identify potential ecological effects. Abstract Genetically engineered (GE) insects have the potential to radically change pest management worldwide. With recent approvals of GE insect releases, there is a need for a synthesized framework to evaluate their potential ecological and evolutionary effects. The effects may occur in two phases: a transitory phase when the focal population changes in density, and a steady state phase when it reaches a new, constant density.
We review potential effects of a rapid change in insect density related to population outbreaks, biological control, invasive species, and other GE organisms to identify a comprehensive list of potential ecological and evolutionary effects of GE insect releases. We apply this framework to the Anopheles gambiae mosquito – a malaria vector being engineered to suppress the wild mosquito population – to identify effects that may occur during the transitory and steady state phases after release.
Introduction Conceptual framework Box 1. Transitory Phase Gene flow Box 2. Bull World Health Organ 2016;94:766–771 Use of transgenic Aedes aegypti in Brazil: risk perception and assessment. PLOS 06/10/16 What Is Stopping the Use of Genetically Modified Insects for Disease Control? BMJ 27/05/16 Can genetically modified mosquitoes stop Zika? No, but genetically modified bacteria can. REUTERS 05/08/16 GM mosquito trial will not significantly impact environment: FDA.
THE SCIENTIST 05/08/16 FDA: GM Mosquitoes Safe for Environment. PLOS 02/07/15 Suppression of a Field Population of Aedes aegypti in Brazil by Sustained Release of Transgenic Male Mosquitoes. Abstract. MEM INST OSWALDO CRUZ, RIO DE JANEIRO, 109(5) August 2014 Metagenomics, paratransgenesis and the Anopheles microbiome:a portrait of the geographical distribution of the anopheline microbiota based on a meta-analysis of reported taxa. CDC EID – FEV 2015 - Awareness and Support of Release of Genetically Modified “Sterile” Mosquitoes, Key West, Florida, USA. Author affiliations: University of Arizona, Tucson, Arizona, USA (K.C. Ernst, S. Haenchen, K. Walker); National Center of Atmospheric Research, Boulder, Colorado, USA (K. PLOS 13/03/14 A Regulatory Structure for Working with Genetically Modified Mosquitoes: Lessons from Mexico. Citation: Ramsey JM, Bond JG, Macotela ME, Facchinelli L, Valerio L, Brown DM, et al. (2014) A Regulatory Structure for Working with Genetically Modified Mosquitoes: Lessons from Mexico.
PAHO - AOUT 2014 - Technical note on transgenic mosquitoes engineered for Aedes aegypti control. BMC 23/06/15 A delivery system for field application of paratransgenic control. Bacterial culture P. agglomerans E325 culture was maintained on Luria Bertani (LB) agar at 30 °C or in LB broth at 30 °C at 180 rpm. OMS 26/06/14 A new framework for evaluating genetically modified mosquitoes. Insects 2015, 6(1), 236-278 Fighting Arbovirus Transmission: Natural and Engineered Control of Vector Competence in Aedes Mosquitoes.
CDC EID – FEV 2015 - Awareness and Support of Release of Genetically Modified “Sterile” Mosquitoes, Key West, Florida, USA. ISAUDE 01/07/13 Largest project in the world loose 430 million modified Aedes aegypti in BahiaWill be released into the environm. A new stage design Transgenic Aedes (PAT), which frees males genetically modified to combat wild insect, that transmits dengue, was started in the city of Bahia Jacobina. Will be released approximately 4 million mosquitoes a week for the next three years to combat dengue. Result of an agreement between the Institute of Biomedical Sciences (ICB) and the USP biofactory Moscamed Brazil, PAT will release in this new stage, more than 430 million mosquitoes.
"This is a pioneering project in the world. Nowhere was made a release of transgenic mosquitoes that size, the researcher afima ICB, Margareth Lara Capurro, coordinator of PAT. Lineage insect used was developed by British scientists Company Oxitec, partner in the project, and is prouzida Unit Production of Transgenic Aedes Moscamed in Juazeiro, today with a weekly production of approximately 1 million males. OBC/wprs Bulletin Vol. 73, 2012 Defining environmental risk assessment criteria for GM insects to be placed on the EU market. Parasites & Vectors 2011, 4:82 (19 May 2011) The paratransgenic sand fly: A platform for control of Leishmania transmission. THE NEW YORKER 09/07/12 The Mosquito Solution - Can genetic modification eliminate a deadly tropical disease? Rev. Inst. Med. trop. S. Paulo vol.54 no.5 São Paulo Sept./Oct. 2012 Genetic Control of Mosquitoes: population suppression strat.
Rev. Inst. Med. trop. S. Paulo vol.54 no.5 São Paulo Sept./Oct. 2012 Genetic Control of Mosquitoes: population suppression strategies – guatemalt
Peer Reviewed Journal 03/01/13 Transgenic sexing system for Ceratitis capitata (Diptera: Tephritidae) based on female-specific e.
Peer Reviewed Journal 03/01/13 Transgenic sexing system for Ceratitis capitata (Diptera: Tephritidae) based on female-specific embryonic lethality – guatemalt
Int J Biol Sci 2011; 7:1334-1344 Paratransgenic Control of Vector Borne Diseases 1. Institute of Dentistry, Queen Mary, Univers. INTECH - FEV 2012 - Transgenesis, Paratransgenesis and Transmission Blocking Vaccines to Prevent Insect-Borne Diseases. PLOS 21/04/11 Engineered Resistance to Plasmodium falciparum Development in Transgenic Anopheles stephensi.
Abstract Transposon-mediated transformation was used to produce Anopheles stephensi that express single-chain antibodies (scFvs) designed to target the human malaria parasite, Plasmodium falciparum. The scFvs, m1C3, m4B7, and m2A10, are derived from mouse monoclonal antibodies that inhibit either ookinete invasion of the midgut or sporozoite invasion of salivary glands. The scFvs that target the parasite surface, m4B7 and m2A10, were fused to an Anopheles gambiae antimicrobial peptide, Cecropin A. Previously-characterized Anopheles cis-acting DNA regulatory elements were included in the transgenes to coordinate scFv production with parasite development.