J Med Entomol. 2012 Nov;49(6):1177-88. Mathematical models as aids for design and development of experiments: the case of transgenic mosquitoes. SCIENCEMAG 17/09/19 Study on DNA spread by genetically modified mosquitoes prompts backlash. For 10 years, the company Oxitec has been testing whether genetically modified (GM) mosquitoes can suppress populations of their natural brethren, which carry devastating viruses such as Zika and dengue.
Its strategy: Deploy (nonbiting) male Aedes aegypti mosquitoes bearing a gene that should doom most of their offspring before adulthood. Now, a team of independent researchers analyzing an early trial of Oxitec’s technology is raising alarm—and drawing fire from the firm—with a report that some offspring of the GM mosquitoes survived and produced offspring that also made it to sexual maturity. As a result, local mosquitoes inherited pieces of the genomes of the GM mosquitoes, the team revealed last week in Scientific Reports. There’s no evidence that these hybrids endanger humans more than the wild mosquitoes or that they’ll render Oxitec’s strategy ineffective, both the paper’s authors and the company agree. FRESH FRUIT PORTAL 29/01/20 First open-field trial of GM moth 'could herald new era of crop protection' A new study reports a successful, first-ever open-field release of a self-limiting, genetically engineered diamondback moth.
Researchers believe that the results of the study pave the way for an effective and sustainable approach to pest control. The diamondback moth, also known as Plutella xylostella, is highly damaging to brassica crops such as cabbage, broccoli, cauliflower and canola. Front. Bioeng. Biotechnol., 29 January 2020 First Field Release of a Genetically Engineered, Self-Limiting Agricultural Pest Insect: Evaluating Its Potential for Future Crop Protection. Introduction Arthropod pests cause an estimated >$470 billion in lost agricultural crops worldwide (Culliney, 2014).
The main tool for controlling such pests is the use of insecticides, the global annual market value of which is projected to reach $16.44 billion by 2019 (Statistica, 2019). Insecticides will remain an important component of integrated pest management (IPM) programs but there are concerns about their off-target effects. Furthermore, resistance to insecticides is a growing problem, with 586 insect species known to be resistant to one or more insecticides (Sparks and Nauen, 2015).
BMC MICROBIOLOGY 23/11/18 Towards improving tsetse fly paratransgenesis: stable colonization of Glossina morsitans morsitans with genetically modified Sodalis. As vector-borne diseases continue to present significant threats to human, animal and plant health, there is a perpetual need to improve existing and develop new control strategies. Vector control has always been and still remains a key component of any integrated program to control vector-borne diseases.
In the light of increasing insecticide resistance combined with the growing awareness of the negative environmental and ecological consequences caused by the widespread use of insecticides, genetic control of the insect vector provides a valuable addition to the armory of tools already available. Tsetse flies (Glossina sp.) are medically and agriculturally important vectors that transmit Trypanosoma spp. parasites responsible for human sleeping sickness and animal African trypanosomiasis (AAT). UNICAMP_BR - 2019 - Dissertation en ligne : Dengue's fluctuation in a middle city, with genetically modified mosquitoes liberation : a longitudinal study. TRENDS IN PARASITOLOGY - JUNE 2018 - Transgenic Mosquitoes – Fact or Fiction? THECONVERSATION 20/08/18 Genetically modified mosquitoes may be best weapon for curbing disease transmission. Mosquitoes are some of the most deadly creatures on the planet.
They carry viruses, bacteria and parasites, which they transmit through bites, infecting some 700 million people and killing more than 1 million each year. With international travel, migration and climate change, these infections are no longer confined to tropical and subtropical developing countries. Pathogens such as West Nile virus and Zika virus have caused significant outbreaks in the United States and its territories that are likely to continue, with new invasive pathogens being discovered all the time.
Currently, control of these diseases is mostly limited to broad-spectrum insecticide sprays, which can harm both humans and non-target animals and insects. PNAS 26/02/19 Engineered resistance to Zika virus in transgenic Aedes aegypti expressing a polycistronic cluster of synthetic small RNAs. PARASITES & VECTORS 21/12/18 Molecular tools and genetic markers for the generation of transgenic sexing strains in Anopheline mosquitoes. NATURE 08/10/18 Blood meal-induced inhibition of vector-borne disease by transgenic microbiota.
IFLSCIENCE 17/04/19 How Genetically Edited Doublesex Mosquitoes Could Save Millions Of Lives. Syndicated from the blog of Bill Gates.
Bill Gates is a financial sponsor of IFLScience. It’s Mosquito Week again on the Gates Notes. This year I’m exploring some of the science behind malaria and other mosquito-borne diseases. You can read below about how gene editing could play a key role in eradicating malaria. I’ve also written about amazing advances in tracking the disease and how the parasite is a deadly shapeshifter. Humans have spent thousands of years inventing new ways to kill mosquitoes. But evolution is smart. This is especially problematic for the fight against mosquito-borne diseases like malaria. Our foundation is backing a lot of different advances. What is cool about these genetic techniques is how precise they can be. AFRICAN CENTRE FOR BIODIVERSITY 02/05/19 STOP RISKY GM MOSQUITO RELEASES – WE HAVE THE RIGHT TO SAY NO. We, the undersigned civil society organisations in Africa, hereby call upon the Bill and Melinda Gates Foundation and the Target Malaria project to stop the intended release of 10 000 genetically modified (GM) “male sterile” mosquitoes in Burkina Faso, as the release poses unacceptable risks to human beings and the environment.
We note with grave concern that African citizens are being exposed to life threatening health risks, through questionable technology involving the release of GM mosquitoes. We are even more alarmed to learn that, by Target Malaria’s own admission, there are no advantages expected from the initial proposed GM mosquito release, as it is intended only for training purposes and not expected to deliver any benefits for malaria control in Burkina Faso. There is absolutely no justification for releasing these GM mosquitoes in Burkina Faso or elsewhere on the continent. CORNELL ALLIANCE FOR SCIENCE 05/09/17 First US field trials under way for self-limiting "GMO" insect.
Scientists at Cornell University have confirmed that they have begun releasing genetically engineered diamondback moths into an isolated field on a research farm in upstate New York.
The focus of the project is to investigate the potential for biotechnology to reduce insecticide use in vegetable farming. Diamondback moths, a serious pest of cabbage and other brassica vegetable and field crops, are an invasive pest in the United States. Around the world they cause billions of dollars worth of damage to cabbage, kale, broccoli, cauliflower and canola plants each year, forcing farmers to spray insecticides in order to protect their crops. The Cornell project involves the targeted release of genetically engineered male diamondback moths carrying a self-limiting gene.
These males seek out and mate with wild-type females. Scientists hope that by using this environmentally-friendly technique farmers can reduce insecticide use on their crops. Vector-Borne and Zoonotic Diseases 01/01/18 Studies of Transgenic Mosquitoes in Disease-Endemic Countries: Preparation of Containment Facilities. PLOS 26/07/18 Genetically engineered mosquitoes, Zika and other arboviruses, community engagement, costs, and patents: Ethical issues. Citation: Meghani Z, Boëte C (2018) Genetically engineered mosquitoes, Zika and other arboviruses, community engagement, costs, and patents: Ethical issues.
PLoS Negl Trop Dis 12(7): e0006501. VECTOR-BORNE AND ZOONOTIC DISEASES 01/01/18 Containment Studies of Transgenic Mosquitoes in Disease Endemic Countries: The Broad Concept of Facilities Readiness. Labiotech.eu VIA YOUTUBE 21/06/17 Synbio: Genetically modified mosquitos against diseases - Hadyn Parry, Oxitec's CEO. Social Epistemology - 2018 - Matters of Dwelling: Releasing the Genetically Engineered Aedes Aegypti Mosquito in Key West. VECTOR-BORNE AND ZOONOTIC DISEASES - JANV 2018 - Studies of Transgenic Mosquitoes in Disease-Endemic Countries: Preparation of Containment Facilities. About This Journal...
To cite this article:Quinlan M. Megan, Mutunga James Mutuku, Diabaté Abdoulaye, Namountougou Moussa, Coulibaly Mamadou B., Sylla Lakamy, Kayondo Jonathan, Balyesima Victor, Clark Lorna, Benedict Mark Q., and Raymond Peter. Vector-Borne and Zoonotic Diseases. January 2018, 18(1): 21-30. Published in Volume: 18 Issue 1: January 1, 2018 M. 1Centre for Environmental Policy, Imperial College London, Ascot, United Kingdom. 2International Centre of Insect Physiology and Ecology, Mbita Point, Kenya. 3Department of Biological Sciences, Mount Kenya University, Thika, Kenya. 4Institut de Recherche en Sciences de la Santé, (IRSS)/Centre Muraz, Bobo Dioulasso, Burkina Faso. 5Malaria Research and Training Center, Université des Sciences, des Techniques et des Technologies de Bamako (MRTC/USTTB), Bamako, Mali. 6Uganda Virus Research Institute (UVRI), Entebbe, Uganda. 7Department of Life Sciences, Imperial College London, Ascot, United Kingdom. 9Donald Danforth Plant Science Center, St.
VECTOR-BORNE AND ZOONOTIC DISEASES - JANV 2018 - Maintaining Quality of Candidate Strains of Transgenic Mosquitoes for Studies in Containment Facilities in Disease Endemic Countries s9" About This Journal...
To cite this article:Mumford John D., Leach Adrian W., Benedict Mark Q., Facchinelli Luca, and Quinlan M. Megan. Vector-Borne and Zoonotic Diseases. EUREKALERT 28/09/17 Disease resistance successfully spread from modified to wild mosquitoes. WHAT: Using genetically modified (GM) mosquitoes to reduce or prevent the spread of infectious diseases is a new but rapidly expanding field of investigation. Among the challenges researchers face is ensuring that GM mosquitoes can compete and mate with their wild counterparts so the desired modification is preserved and spread in the wild population.
Investigators at Johns Hopkins University have engineered GM mosquitoes to have an altered microbiota that suppresses human malaria-causing parasites. These GM mosquitos preferred to mate with wild mosquitoes and passed along the desired protection to many generations of offspring. The research was funded by the National Institute of Allergy and Infectious Diseases (NIAID), part of the National Institutes of Health. The researchers genetically modified Anopheles mosquitoes, which in nature spread the malaria-causing parasite Plasmodium.
A. To schedule interviews, please contact Ken Pekoc, (301) 402-1663, email@example.com. NIH 28/09/17 Disease resistance successfully spread from modified to wild mosquitoes NIAID-funded group assesses mating of genetically modified species. INTECH - AVRIL 2017 - Biological Control of Pest and Vector Insects Au sommaire notamment : Developing the Arsenal Against Pest and Vector Dipterans: Inputs of Transgenic and Paratransgenic Biotechnologies. David Bethune VIA YOUTUBE 15/09/16 Miami Used Naled Spraying to Hide GMO Mosquito Agenda. David Bethune VIA YOUTUBE 10/09/16 Citizens Defeat GMO Mosquito Resolution (Part 1) Al Jazeera English via YOUTUBE 04/12/16 Can genetically-modified mosquitoes help eradicate malaria? - TechKnow. FDA 05/08/16 Environmental Assessment for Investigational Use of Aedes aegypti OX513A. ECOLOGY AND EVOLUTION 12/09/13 Release of genetically engineered insects: a framework to identify potential ecological effects.
Abstract Genetically engineered (GE) insects have the potential to radically change pest management worldwide. With recent approvals of GE insect releases, there is a need for a synthesized framework to evaluate their potential ecological and evolutionary effects. The effects may occur in two phases: a transitory phase when the focal population changes in density, and a steady state phase when it reaches a new, constant density. We review potential effects of a rapid change in insect density related to population outbreaks, biological control, invasive species, and other GE organisms to identify a comprehensive list of potential ecological and evolutionary effects of GE insect releases.
We apply this framework to the Anopheles gambiae mosquito – a malaria vector being engineered to suppress the wild mosquito population – to identify effects that may occur during the transitory and steady state phases after release. Introduction Conceptual framework Box 1. Transitory Phase Gene flow Box 2. Bull World Health Organ 2016;94:766–771 Use of transgenic Aedes aegypti in Brazil: risk perception and assessment. PLOS 06/10/16 What Is Stopping the Use of Genetically Modified Insects for Disease Control? Citation: Panjwani A, Wilson A (2016) What Is Stopping the Use of Genetically Modified Insects for Disease Control?
PLoS Pathog 12(10): e1005830. doi:10.1371/journal.ppat.1005830 Editor: Carolyn B. Coyne, University of Pittsburgh, UNITED STATES. BMJ 27/05/16 Can genetically modified mosquitoes stop Zika? No, but genetically modified bacteria can. REUTERS 05/08/16 GM mosquito trial will not significantly impact environment: FDA. THE SCIENTIST 05/08/16 FDA: GM Mosquitoes Safe for Environment. PLOS 02/07/15 Suppression of a Field Population of Aedes aegypti in Brazil by Sustained Release of Transgenic Male Mosquitoes. Abstract The increasing burden of dengue, and the relative failure of traditional vector control programs highlight the need to develop new control methods. SIT using self-limiting genetic technology is one such promising method. A self-limiting strain of Aedes aegypti, OX513A, has already reached the stage of field evaluation. Sustained releases of OX513A Ae. aegypti males led to 80% suppression of a target wild Ae. aegypti population in the Cayman Islands in 2010.
Here we describe sustained series of field releases of OX513A Ae. aegypti males in a suburb of Juazeiro, Bahia, Brazil. Author Summary Dengue is a major mosquito-borne disease, increasing in prevalence and severity; there are no specific drugs or licensed vaccine. MEM INST OSWALDO CRUZ, RIO DE JANEIRO, 109(5) August 2014 Metagenomics, paratransgenesis and the Anopheles microbiome:a portrait of the geographical distribution of the anopheline microbiota based on a meta-analysis of reported taxa. CDC EID – FEV 2015 - Awareness and Support of Release of Genetically Modified “Sterile” Mosquitoes, Key West, Florida, USA. Author affiliations: University of Arizona, Tucson, Arizona, USA (K.C. PLOS 13/03/14 A Regulatory Structure for Working with Genetically Modified Mosquitoes: Lessons from Mexico.
PAHO - AOUT 2014 - Technical note on transgenic mosquitoes engineered for Aedes aegypti control. BMC 23/06/15 A delivery system for field application of paratransgenic control. OMS 26/06/14 A new framework for evaluating genetically modified mosquitoes. Insects 2015, 6(1), 236-278 Fighting Arbovirus Transmission: Natural and Engineered Control of Vector Competence in Aedes Mosquitoes. CDC EID – FEV 2015 - Awareness and Support of Release of Genetically Modified “Sterile” Mosquitoes, Key West, Florida, USA. ISAUDE 01/07/13 Largest project in the world loose 430 million modified Aedes aegypti in BahiaWill be released into the environm. A new stage design Transgenic Aedes (PAT), which frees males genetically modified to combat wild insect, that transmits dengue, was started in the city of Bahia Jacobina. Will be released approximately 4 million mosquitoes a week for the next three years to combat dengue.
Result of an agreement between the Institute of Biomedical Sciences (ICB) and the USP biofactory Moscamed Brazil, PAT will release in this new stage, more than 430 million mosquitoes. "This is a pioneering project in the world. OBC/wprs Bulletin Vol. 73, 2012 Defining environmental risk assessment criteria for GM insects to be placed on the EU market.
Parasites & Vectors 2011, 4:82 (19 May 2011) The paratransgenic sand fly: A platform for control of Leishmania transmission. THE NEW YORKER 09/07/12 The Mosquito Solution - Can genetic modification eliminate a deadly tropical disease? Rev. Inst. Med. trop. S. Paulo vol.54 no.5 São Paulo Sept./Oct. 2012 Genetic Control of Mosquitoes: population suppression strat.
Rev. Inst. Med. trop. S. Paulo vol.54 no.5 São Paulo Sept./Oct. 2012 Genetic Control of Mosquitoes: population suppression strategies – guatemalt
Peer Reviewed Journal 03/01/13 Transgenic sexing system for Ceratitis capitata (Diptera: Tephritidae) based on female-specific e. Int J Biol Sci 2011; 7:1334-1344 Paratransgenic Control of Vector Borne Diseases 1. Institute of Dentistry, Queen Mary, Univers. INTECH - FEV 2012 - Transgenesis, Paratransgenesis and Transmission Blocking Vaccines to Prevent Insect-Borne Diseases. PLOS 21/04/11 Engineered Resistance to Plasmodium falciparum Development in Transgenic Anopheles stephensi. Abstract Transposon-mediated transformation was used to produce Anopheles stephensi that express single-chain antibodies (scFvs) designed to target the human malaria parasite, Plasmodium falciparum.
The scFvs, m1C3, m4B7, and m2A10, are derived from mouse monoclonal antibodies that inhibit either ookinete invasion of the midgut or sporozoite invasion of salivary glands. The scFvs that target the parasite surface, m4B7 and m2A10, were fused to an Anopheles gambiae antimicrobial peptide, Cecropin A. Previously-characterized Anopheles cis-acting DNA regulatory elements were included in the transgenes to coordinate scFv production with parasite development.