UCLV_EDU_CU 06/07/17 Thèse en ligne : Optimización de la transformación genética vía Agrobacterium tumefaciens (Smith y Townsend) Conn en Phaseolus vulgaris. Rev. biol. trop vol.67 n.2 San José Apr. 2019 Agrobacterium tumefaciens-mediated transformation of common bean (Phaseolus vulgaris) var. Brunca. References.
PLANT PATHOLOGY 11/03/20 Induction of systemic resistance to Agrobacterium tumefaciens by endophytic bacteria in grapevine. INTECH 14/12/16 New Approaches to Agrobacterium tumefaciens-Mediated Gene Transfer to Plants. 1.
Introduction Genetic engineering could use the genetic resources that exist in nature without any limitation. With the use of these techniques, a gene cloned to an organism from any living being (human, animal, plant, and microorganisms) could be easily transferred. For instance, a gene cloned from bacteria could be placed in the plant cell to give resistance to the pests, and this transfer gives an opportunity for the production of the organisms expressed as “genetically modified” or transgenic.
These transferred genes replicate with the natural plant genes after they are placed in the organism and produce protein. The basis of the techniques used for gene transfer to plants is the integration of a DNA segment including the gene of interest into chromosomes of the plant cells and thereof the recovery of transgenic plants from transformed cells by using tissue culture methods. Genet. Mol. Biol. vol.42 no.2 Ribeirão Preto Apr./June 2019 Synthetic and minimalist vectors for Agrobacterium tumefaciens-mediated transformation of fungi.
References.
FRONT. PLANT SCI. 23/04/14 Plant responses to Agrobacterium tumefaciens and crown gall development. Introduction Agrobacterium tumefaciens causes crown gall disease on a wide range of host species by transferring and integrating a part of its own DNA, the T-DNA, into the plant genome (Chilton et al., 1977).
This unique mode of action has also made the bacterium an important tool in plant breeding. After attachment of Agrobacterium to plant cells and expression of multiple virulence (vir) genes, several effector proteins, together with T-DNA, are transported into the plant cell by a type-IV-secretion system (Thompson et al., 1988; Ward et al., 1988, 2002; Kuldau et al., 1990; Shirasu et al., 1990; Beijersbergen et al., 1994). Plant factors assist with T-DNA integration into the plant genome (Gelvin, 2000; Mysore et al., 2000; Tzfira et al., 2004; Magori and Citovsky, 2012). After integration, expression of the T-DNA-encoded oncogenes iaaH, iaaM, and ipt induces biosynthesis of auxin and cytokinin (Morris, 1986; Binns and Costantino, 1998).
Pathogen Defense Phytohormones. Braz. arch. biol. technol. vol.44 no.2 Curitiba June 2001 An Improved Method for Transformation of Lettuce by Agrobacterium tumefaciens with a Gene that Confers Freezing Resistance. An Improved Method for Transformation of Lettuce by Agrobacterium tumefaciens with a Gene that Confers Freezing Resistance Marcos Pileggi1*, Albanin Aparecida Mielniczki Pereira1, Joandrei dos Santos Silva1, Sônia Alvim Veiga Pileggi1 and Desh Pal S.
Verma2 1Departamento de Biologia Geral, Setor de Ciências Biológicas e da Saúde, Universidade Estadual de Ponta Grossa, Campus Uvaranas, Av. Carlos Cavalcanti, 4748, 84030-000, Ponta Grossa, PR, Brazil; 2Plant Biotechnology Center, Ohio State University, 1060 Carmack Road, Columbus, OH 43210, USA An efficient method for constructing transgenic lettuce cultivars by Agrobacterium tumefaciens was described by Torres et al., 1993. In the present work, an improvement of the above procedure is described and applied to transform the cultivar Grand Rapids with a mutated P5CS gene. Key words: Stress resistance; cold resistance; transgenic; lettuce; proline The lettuce (Lactuca sativa L.) cultivar Grand Rapids was used in this work.
BMC PLANT BIOLOGY 26/11/18 Targeted mutagenesis using the Agrobacterium tumefaciens-mediated CRISPR-Cas9 system in common wheat. 1.Jiang W, Zhou H, Bi H, Fromm M, Yang B, Weeks DP.
Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice. Nucleic Acids Res. 2013;41(20):e188.CAS Article Google Scholar 2.Li JF, Norville JE, Aach J, McCormack M, Zhang D, Bush J, Church GM, Sheen J. Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9. AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH 18/03/11 Sensitive and specific detection of Agrobacterium tumefaciens in soil using a rapid polymerase chain reaction (PCR) In: CONGRESSO INTERINSTITUCIONAL DE INICIAÇÃO CIENTÍFICA (BR) - 2019 - Poster : Transformação genética de milho por Agrobacterium tumefaciens. Louisiana State University - AOUT 2006 - Thèse en ligne : AGROBACTERIUM TUMEFACIENS-MEDIATED TRANSFORMATION OF TOBACCO (NICOTIANA TABACUM L.) LEAF DISKS: EVALUATION OF THE CO-CULTIVATION CONDITIONS TO INCREASE β-GLUCURONIDASE GENE ACTIVITY. EJB Electronic Journal of Biotechnology - 1998 - Agrobacterium tumefaciens: a natural tool for plant transformation.
Agrobacterium tumefaciens: a natural tool for plant transformation Gustavo A. de la Riva* Laboratory of Environmental Biotechnology.
Plant Division. Centre for Genetic Engineering and Biotechnology (CIGB). WIKIPEDIA ANGLOPHONE - Agrobacterium tumefaciens. Agrobacterium tumefaciens (updated scientific name: Rhizobium radiobacter)[2][3] is the causal agent of crown gall disease (the formation of tumours) in over 140 species of Eudicot.
It is a rod shaped, Gram negative soil bacterium.[1] Symptoms are caused by the insertion of a small segment of DNA (known as the T-DNA, for 'transfer DNA'), from a plasmid, into the plant cell,[4] which is incorporated at a semi-random location into the plant genome. Agrobacterium tumefaciens (or A. tumefaciens) is an alphaproteobacterium of the family Rhizobiaceae, which includes the nitrogen fixing legume symbionts. Unlike the nitrogen fixing symbionts, tumor producing Agrobacterium are pathogenic and do not benefit the plant. The wide variety of plants affected by Agrobacterium makes it of great concern to the agriculture industry.[5] Conjugation[edit] Method of infection[edit] WIKIPEDIA - Agrobacterium tumefaciens. Un article de Wikipédia, l'encyclopédie libre.
Le mécanisme de formation des galles s'apparente à une transformation génétique, il est dû à un plasmide bactérien appelé plasmide Ti qui rend les bactéries virulentes. Un fragment d'ADN du plasmide Ti, l'ADN-T est transféré de la bactérie vers la plante, puis intégré dans le génome végétal où il induit la formations des galles caractérisés par la multiplication anarchique des cellules végétales. Agrobacterium tumefaciens infecte essentiellement les dicotylédones à la faveur d'une blessure. Cette observation constitue le fondement d'une des techniques les plus utilisées en ingénierie génétique des végétaux pour produire des organismes génétiquement modifiés. Génome[modifier | modifier le code] Cycle infectieux[modifier | modifier le code] AGRIRESEAU - La tumeur du collet et de la tige causée par Agrobacterium. AGROSCOPE DE CHANGINS - Tumeur bactérienne du collet et des racines.
CNRS 19/10/07 La bactérie Agrobacterium tumefaciens utilise le système de défense des plantes pour les transformer. La majorité des tumeurs que l'on peut observer chez les plantes sont causées par les bactéries de l'espèce Agrobacterium tumefaciens.
Elles ont la faculté unique de transformer les plantes par injection d'un fragment de leur ADN dans le génome des plantes, l'ADN-T. Des gènes bactériens sont ainsi transférés à la plante et stimulent la division cellulaire, provoquant ainsi la formation de tumeurs. Les cellules tumorales ainsi modifiées produisent des acides aminés modifiés, éléments nécessaires à la bactérie pour se multiplier dans la tumeur. JUSSIEU - FEV 2003 - La transgenèse grâce à Agrobacterium tumefaciens. La transgenèse grâce à Agrobacterium tumefaciens Marie Weidner et Gilles Furelaud Article publié en février 2003 La transgenèse consiste à ajouter un nouveau gène dans un organisme. Chez les végétaux, plusieurs techniques de transgénèse ont été développées. La possibilité de régénérer une plante entière à partir de quelques cellules végétales est d'un grand intérêt lors de ces transgénèses.
Une des techniques les plus utilisées en transgenèse végétale est l'utilisation d'une bactérie du sol, Agrobacterium tumefaciens. Mol Plant Pathol. 2012 Aug;13(6):614-29. Top 10 plant pathogenic bacteria in molecular plant pathology.