PLOS 24/03/17 Rapid Surveillance for Vector Presence (RSVP): Development of a novel system for detecting Aedes aegypti and Aedes albopictus. Abstract Background The globally important Zika, dengue and chikungunya viruses are primarily transmitted by the invasive mosquitoes, Aedes aegypti and Aedes albopictus.
In Australia, there is an increasing risk that these species may invade highly urbanized regions and trigger outbreaks. We describe the development of a Rapid Surveillance for Vector Presence (RSVP) system to expedite presence- absence surveys for both species. Methodology/Principal findings We developed a methodology that uses molecular assays to efficiently screen pooled ovitrap (egg trap) samples for traces of target species ribosomal RNA. PLOS 23/03/17 The relationship between entomological indicators of Aedes aegypti abundance and dengue virus infection. PLOS 01/09/16 Genetic Evidence of Expansion by Passive Transport of Aedes (Stegomyia) aegypti in Eastern Argentina. Citation: Díaz-Nieto LM, Chiappero MB, Díaz de Astarloa C, Maciá A, Gardenal CN, Berón CM (2016) Genetic Evidence of Expansion by Passive Transport of Aedes (Stegomyia) aegypti in Eastern Argentina.
PLoS Negl Trop Dis 10(9): e0004839. doi:10.1371/journal.pntd.0004839 Editor: Christopher M. Barker, University of California, Davis, UNITED STATES Published: September 1, 2016 Copyright: © 2016 Díaz-Nieto et al. Funding: This study was funded by Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT PICT-2013-0431), Universidad Nacional de Mar del Plata (15/E692-EXA742) and CONICET (PIP 2012 No 112 20110100963). Competing interests: The authors have declared that no competing interests exist. PLOS 02/02/17 Collective behavior quantification on human odor effects against female Aedes aegypti mosquitoes—Open source development. Abstract Classifying and quantifying mosquito activity includes a plethora of categories, ranging from measuring flight speeds, repellency, feeding rates, and specific behaviors such as home entry, swooping and resting, among others.
Entomologists have been progressing more toward using machine vision for efficiency for this endeavor. Digital methods have been used to study the behavior of insects in labs, for instance via three-dimensional tracking with specialized cameras to observe the reaction of mosquitoes towards human odor, heat and CO2, although virtually none was reported for several important fields, such as repellency studies which have a significant need for a proper response quantification. However, tracking mosquitoes individually is a challenge and only limited number of specimens can be studied. Editor: Jiang-Shiou Hwang, National Taiwan Ocean University, TAIWAN. PLOS 26/04/16 Spread of the Invasive Mosquitoes Aedes aegypti and Aedes albopictus in the Black Sea Region Increases Risk of Chikungunya, Dengue, and Zika Outbreaks in Europe. Citation: Akiner MM, Demirci B, Babuadze G, Robert V, Schaffner F (2016) Spread of the Invasive Mosquitoes Aedes aegypti and Aedes albopictus in the Black Sea Region Increases Risk of Chikungunya, Dengue, and Zika Outbreaks in Europe.
PLoS Negl Trop Dis 10(4): e0004664. doi:10.1371/journal.pntd.0004664 Editor: Roberto Barrera, Centers for Disease Control and Prevention, Puerto Rico, UNITED STATES Published: April 26, 2016 Copyright: © 2016 Akiner et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. PLOS 20/11/15 Deltamethrin Resistance Mechanisms in Aedes aegypti Populations from Three French Overseas Territories Worldwide.
Abstract Background Aedes aegypti is a cosmopolite mosquito, vector of arboviruses.
The worldwide studies of its insecticide resistance have demonstrated a strong loss of susceptibility to pyrethroids, the major class of insecticide used for vector control. French overseas territories such as French Guiana (South America), Guadeloupe islands (Lesser Antilles) as well as New Caledonia (Pacific Ocean), have encountered such resistance. Methodology/Principal Findings We initiated a research program on the pyrethroid resistance in French Guiana, Guadeloupe and New Caledonia. PLOS 23/07/15 Application of wMelPop Wolbachia Strain to Crash Local Populations of Aedes aegypti.
Abstract The endosymbiotic bacteria Wolbachia pipientis (wMel strain) has been successfully established in several populations of Aedes aegypti, the primary dengue vector.
The virulent Wolbachia strain wMelPop is known to cause several pathological impacts (increased egg mortality, life shortening, etc.) reducing overall fitness in the mosquito Ae. aegypti. Increased egg mortality could substantially reduce egg banks in areas with a lengthy monsoonal dry season, and be employed to eliminate local populations.
We tested this application under semi-field cage conditions. First, we determined that wMelPop infection significantly reduced the survival of desiccation-resistant eggs of the dengue vector Ae. aegypti, with shade and temperature having a significant impact; nearly all wMelPop-infected eggs failed to hatch after 6 and 10 weeks in summer and winter conditions, respectively. Author Summary Dengue is a leading cause of morbidity in the tropics. Editor: Jason L. PLOS 24/03/14 Differential Susceptibility of Two Field Aedes aegypti Populations to a Low Infectious Dose of Dengue Virus. Abstract Background The infectious dose required to infect mosquito vectors when they take a blood meal from a viremic person is a critical parameter underlying the probability of dengue virus (DENV) transmission.
Because experimental vector competence studies typically examine the proportion of mosquitoes that become infected at intermediate or high DENV infectious doses in the blood meal, the minimum blood meal titer required to infect mosquitoes is poorly documented. Understanding the factors influencing the lower infectiousness threshold is epidemiologically significant because it determines the transmission potential of humans with a low DENV viremia, possibly including inapparent infections, and during the onset and resolution of the viremic period of acutely infected individuals. PLOS 11/12/14 The Effect of Virus-Blocking Wolbachia on Male Competitiveness of the Dengue Vector Mosquito, Aedes aegypti. Abstract Background The bacterial endosymbiont Wolbachia blocks the transmission of dengue virus by its vector mosquito Aedes aegypti, and is currently being evaluated for control of dengue outbreaks.
Wolbachia induces cytoplasmic incompatibility (CI) that results in the developmental failure of offspring in the cross between Wolbachia-infected males and uninfected females. This increases the relative success of infected females in the population, thereby enhancing the spread of the beneficial bacterium. However, Wolbachia spread via CI will only be feasible if infected males are sufficiently competitive in obtaining a mate under field conditions.
PLOS 16/03/15 Risk Factors for the Presence of Chikungunya and Dengue Vectors (Aedes aegypti and Aedes albopictus), Their Altitudinal Distribution and Climatic Determinants of Their Abundance in Central Nepal. Abstract Background The presence of the recently introduced primary dengue virus vector mosquito Aedes aegypti in Nepal, in association with the likely indigenous secondary vector Aedes albopictus, raises public health concerns.
Chikungunya fever cases have also been reported in Nepal, and the virus causing this disease is also transmitted by these mosquito species. PLOS 01/08/13 Genetic Mapping of Specific Interactions between Aedes aegypti Mosquitoes and Dengue Viruses. Abstract Specific interactions between host genotypes and pathogen genotypes (G×G interactions) are commonly observed in invertebrate systems.
Such specificity challenges our current understanding of invertebrate defenses against pathogens because it contrasts the limited discriminatory power of known invertebrate immune responses. Lack of a mechanistic explanation, however, has questioned the nature of host factors underlying G×G interactions. In this study, we aimed to determine whether G×G interactions observed between dengue viruses and their Aedes aegypti vectors in nature can be mapped to discrete loci in the mosquito genome and to document their genetic architecture.
We developed an innovative genetic mapping strategy to survey G×G interactions using outbred mosquito families that were experimentally exposed to genetically distinct isolates of two dengue virus serotypes derived from human patients. Author Summary Figures Copyright: © 2013 Fansiri et al. PLOS 18/04/13 Dual African Origins of Global Aedes aegypti s.l. Populations Revealed by Mitochondrial DNA. Abstract Background Aedes aegypti is the primary global vector to humans of yellow fever and dengue flaviviruses. Over the past 50 years, many population genetic studies have documented large genetic differences among global populations of this species. These studies initially used morphological polymorphisms, followed later by allozymes, and most recently various molecular genetic markers including microsatellites and mitochondrial markers.
In particular, since 2000, fourteen publications and four unpublished datasets have used sequence data from the NADH dehydrogenase subunit 4 mitochondrial gene to compare Ae. aegypti collections and collectively 95 unique mtDNA haplotypes have been found. PLOS 17/01/13 Spatial Patterns of High Aedes aegypti Oviposition Activity in Northwestern Argentina.
PLOS - NOV 2011 - Larval Development of Aedes aegypti and Aedes albopictus in Peri-Urban Brackish Water and Its - Implications f. PLOS 21/12/12 Assessing the Feasibility of Controlling Aedes aegypti with Transgenic Methods: A Model-Based Evaluation. Suppression of dengue and malaria through releases of genetically engineered mosquitoes might soon become feasible. Aedes aegypti mosquitoes carrying a conditionally lethal transgene have recently been used to suppress local vector populations in small-scale field releases. Prior to releases of transgenic insects on a wider scale, however, most regulatory authorities will require additional evidence that suppression will be effective in natural heterogeneous habitats. We use a spatially explicit stochastic model of an Ae. aegypti population in Iquitos, Peru, along with an uncertainty analysis of its predictions, to quantitatively assess the outcome of varied operational approaches for releases of transgenic strains with conditional death of females.
We show that population elimination might be an unrealistic objective in heterogeneous populations. Figures Editor: Nikos Vasilakis, University of Texas Medical Branch, United States of America Copyright: © 2012 Legros et al. Introduction. PLOS 21/12/12 Assessing the Feasibility of Controlling Aedes aegypti with Transgenic Methods: A Model-Based Evaluation. PLOS - NOV 2011 - Larval Development of Aedes aegypti and Aedes albopictus in Peri-Urban Brackish Water and Its Implications for. PLOS 15/11/11 Global Cross-Talk of Genes of the Mosquito Aedes aegypti in Response to Dengue Virus Infection. PLOS 29/03/12 Dengue Virus Infection of the Aedes aegypti Salivary Gland and Chemosensory Apparatus Induces Genes that Modulate. Abstract The female Aedes aegypti salivary gland plays a pivotal role in bloodmeal acquisition and reproduction, and thereby dengue virus (DENV) transmission. It produces numerous immune factors, as well as immune-modulatory, vasodilatory, and anti-coagulant molecules that facilitate blood-feeding.
To assess the impact of DENV infection on salivary gland physiology and function, we performed a comparative genome-wide microarray analysis of the naïve and DENV infection-responsive A. aegypti salivary gland transcriptomes. DENV infection resulted in the regulation of 147 transcripts that represented a variety of functional classes, including several that are essential for virus transmission, such as immunity, blood-feeding, and host-seeking. RNAi-mediated gene silencing of three DENV infection-responsive genes - a cathepsin B, a putative cystatin, and a hypothetical ankyrin repeat-containing protein - significantly modulated DENV replication in the salivary gland.
Author Summary Figures. PLOS 16/03/15 Risk Factors for the Presence of Chikungunya and Dengue Vectors (Aedes aegypti and Aedes albopictus), Their Altitudinal Distribution and Climatic Determinants of Their Abundance in Central Nepal.