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The Clontech In-Fusion ® PCR Cloning System allows you to fuse of the ends of the PCR fragment to the homologous ends of a linearized vector. The 3' and 5' regions of homology are generated by adding 15 bp extensions to both PCR primers that precisely match the ends of the linearized vector. When the vector is combined with your insert, the In-Fusion® enzyme converts the double-stranded extensions into single-stranded DNA and fuses these regions to the corresponding ends of the linearized vector. In-Fusion ® Primer Design Tool converts existing PCR Primers into In-Fusion® primers by adding vector-specific sequences on the 5'-ends of your primers.
Seq and Oligo
Enter or paste a DNA (or RNA) sequence into the upper text box, then click the Translate button. Two types of output are generated: A graphical representation of all six translational frames , showing methionines (ATG) and stop codons. Large open reading frames are readily observed on this map.
Welcome to Webcutter 2.0! This new version of Webcutter is a complete rewrite. Along with cleaner and more maintainable code, I am pleased to introduce the following new features: Rainbow cutters Highlight your favorite enzymes in color or boldface for easy at-a-glance identification Silent cutters Find sites which may be introduced by silent mutagenesis of your coding sequence Sequence uploads Input sequences directly into Webcutter from a file on your hard drive without needing to cut-and-paste Degenerate sequences Analyze restriction maps of sequences containing ambiguous nucleotides like N, Y, and R.