14 FAQs in MS identification. 14 FAQs in MS identification MS technology has an essential role in protein identification and other experiments; however, the current application is not particularly extensive. Therefore I collected some frequently ask questions in the field and offered some solutions, hoping to better help you understand the technology. Questions 1: What is the difference between MS and tandem mass spectrometry? When should I do MS, and when should I apply tandem mass spectrometry? Question 2: what if the species studied is not model organism?
A: You can refer to the closest relationship model organism to make a successful identification of proteins, but if the mass spectra identification results is great without proof of a new protein, then technology such as de-novo can be used. Question 3: How to evaluate the quality of mass spectrometry? Question 4: what’s the purpose of some special mass spectrometry? Question 5: What staining method is better? To be continued in the next blog entry…