Membrane protein co-crystallization. Co-crystals, which retain the unique crystalline structures with their multiple components, have become a very important manipulation in the field of pharmaceuticals (i.e., drug discovery & design).
Creative Biostructure can streamline the procedures of protein preparation, co-crystal generation and structure determination with the highest quality based on our UniCrys™ structural biology platform. Prior to protein complex preparation, biophysical experiments can quantify binding affinities between a protein and its ligands (e.g., proteins, DNA/RNA, chemical compounds, metal ions, etc.). Such quantification can be performed by either our Biophysical Characterization Platform or customers themselves. Types of Components: Substrates Nucleic acids Cofactors Small molecules Ions Heterogenous proteins Biophysical Methods: For co-crystal preparation, strategies like co-crystallization and soaking are employed by our UniCrys™ platform.
Co-crystal Generation Methods: Online Inquiry. Aldehyde dehydrogenase. Creative Biostructure can provide custom MemPro™ gene-to-structure services for aldehyde dehydrogenase.
Aldehyde dehydrogenases (ALDHs), found in all living organisms from Archaea and Eubacteria to eukaryotes, are a large group of polymorphic enzymes that can catalyze the oxidation of aldehydes to carboxylic acids, which leave the liver and are metabolized by the body’s muscle and heart. ALDH also refers to long-chain-aldehyde dehydrogenases, which is encoded by ALDH3 and involved in the removal of toxic aldehyde generated by alcohol metabolism and lipid peroxidation. It is widely expressed on the surface of endoplasm reticulum, and any saturated or unsaturated fatty acids between 6 to 24 carbon lengths can act as its substrates. The human ALDH superfamily includes 19 isozymes that have important physiological and toxicological functions. Membrane protein production. Creative Biostructure provides one-stop MemPro™ gene-to-structure services for membrane protein production, purification, crystallization, structure determination and characterization.
Scientists from Creative Biostructure have rich experience and advanced techniques (e.g., energy-dispersive X-ray spectroscopy, NMR spectroscopy and Cyro-Electron Microscopy) to serve customers with membrane protein structure determination and analysis. Over 50% of modern medical drug targets at membrane proteins, whose structures and functions are largely unknown. However, membrane proteins are known to be difficult to crystallize with traditional methods because of the rich hydrophobic and insoluble features. Computer assisted drug design. Creative Biostructure provides custom virtual screening services based on our unparalleled MagHelix™ structural biology platform.
Given the 3D structure of a protein (by either X-ray crystallography, Electron Microscopy (EM), Nuclear Magnetic Resonance (NMR) or homology modeling), virtual screening searches for compounds that potentially bind with the target protein. Our MagHelix™ virtual screening services can significantly improve the hit rates and reduce the costs (e.g., labor cost) of compound screening. Drug screening. Utilizing multiple technology platforms, Creative Biostructure forms highly responsive teams led by experienced researchers to provide MagHelix™ drug discovery services.
According to your specific demands, our scientists supply customized drug discovery strategies to identify lead compounds and preclinical drug candidates. Figure 1. Drug discovery cycle. Modern drug discovery involves the identification of screening hits, medicinal chemistry and optimization of those hits to increase the affinity, selectivity, efficacy/potency, metabolic stability, and oral bioavailability. Creative biostructure can adopt optimal strategies to reduce the potential of side effects of drugs or increase the half-life of drugs during drug discovery procedure. With strong expertise in medicinal chemistry, biochemistry, molecular and cell biology, our team is dedicated to structural and computational drug design against targets. Increasing activity against the chosen target.
Nuclear magnetic resonance service. NMR is one of the most powerful and versatile non-destructive spectroscopic methods for structure elucidation.
Creative Biostructure has established a state-of-the-art MagHelix™ NMR platform based on spectrometers ranging from 300 MHz to 800 MHz. We provide various NMR service packages depending on your sample types. Both solution- and solid-state samples are acceptable. NMR spectroscopy can obtain detailed information of a molecule’s 3D structure based on its chemical shifts, Zeeman shifts and knight shifts. Scientists from Creative Biostructure have years of experience in the preparation of specimens and development of protein expression systems for high-resolution NMR spectra. Specimens meeting the following requirements can be proceeded for NMR spectroscopy: Typically, Creative Biostructure follows the below SOP to provide custom MagHelix™ NMR services: Online Inquiry.
Biochemical analysis. Bioinformatics and biochemical analysis includes the analysis and prediction of properties related to sequences and structures.
It is as important and necessary as literature investigation before starting a project. Bioinformatics is a practical tool to the analysis of genes and proteins. Information on amino acid compositions, secondary structures, native/homologous structures provides valuable insights for customers. Figure 1. Homologous sequence blast and analysis Multiple sequence alignments are now one of the most widely used bioinformatics analysis.
Figure 2. Phylogenetic analysis of bioinformatics is a fairly straightforward process that can be applied effectively. Biochemical analysis techniques refer to a set of methods, assays, and procedures to analyze the substances found in living organisms and the chemical reactions underlying life processes.