Category:Strains - EcoliWiki. Strain Information of E. coli K12 MG1655. Strain Information Description Genotype: F- lambda- - -50 -1 Serotype: OR:H48:K- This strain was sequenced by the Blattner laboratory because it approximates wild-type and "has been maintained as a laboratory strain with minimal genetic manipulation, having only been cured of the temperate bacteriophage lambda and F plasmid by means of ultraviolet light and acridine orange, respectively. " (Blattner, et al. 1997). MG1655 was derived and named by Mark Guyer from strain W1485, which was derived in Joshua Lederberg's lab from a stab-culture descendant of the original K-12 isolate.
Media and growth curves MG1655 grows on LB and M9 minimal medium (+ Glucose + 1ug/ml thiamine). Recipe for MOPS Rich Defined Medium and MOPS Minimal Medium Growth curve for MG1655 on MOPS Minimal Medium Information and growth curve for a commercially available rich defined medium (MEM alpha w/o phenol red) Growth curves for MG1655 on M9 Minimal Medium. E.coli Database Portal.
PortEco Home Page. E.coli Gene Expression Database (ver 2.0)
Sequencing. What you need to know about OD600. If you use a spec to measure cell density, you may making a very common mistake and taking inaccurate measurements as a result. Specs are often used for measuring the density of suspension cultures, but the mistake that many people make is to record the OD given by the spec as an absolute value. The OD value represents the amount of light that is absorbed by your sample. But that value is affected by the intensity of the light beam in the spec, and the spec design. This means that similar samples will give completely different OD values in different specs due to the specs having different bulbs, or even in the same spec over time, as the beam intensity reduces with the age of the bulb. So recording an OD value in your lab book does not really mean anything as this number is as much dependent on your spec as the density of your culture. What you really want to know What you really want to know from an OD600 reading is the density of the cells e.g. in cells/mL.