Molarity Calculator. Buffer tables. The following are recipes for a number of common biological buffers taken from Ruzin, 1999 Plant Microtechnique and Microscopy. When choosing one for a particular application select a buffer based on its pH optimum and biological properties rather than its historical use. Many buffer species have an impact on biological systems, enzyme activities, substrates, or cofactors (Perrin and Dempsey, 1974). For example, phosphate buffers inhibit the activity of several metabolic enzymes including carboxylase, fumarase, and phosphoglucomutase. Barbiturate uncouples oxidative phosphorylation. Tris buffer reacts with primary amines and modifies electron transport and phosphorylation in chloroplasts.
Tris also inhibits respiratory enzymes in mitochondria. HEPES does not have these negative effects yet buffers at a similar pH range. Combine 25 ml 0.2 M glycine and x ml HCl and dilute to 100 ml with DI (Dawson, et al., 1969). Stock solutions: A 0.2 M NaH2PO4 B 0.2 M Na2HPO4. Protéine : M.M., pI, composition, titrage.
UniProtKB/Swiss-Prot. ProtParam tool. Psipred : Protein Sequence Analysis Workbench. The PSIPRED Protein Sequence Analysis Workbench aggregates several UCL structure prediction methods into one location. Users can submit a protein sequence, perform the predictions of their choice and receive the results of the prediction via e-mail or the web. For a summary of the available methods you can read More... NOTE: users who need to run our methods on a large number of proteins should consider downloading our software using the menu on the left (Server Navigation -> Software Download).
The PSIPRED Team Current Contributors David T. Jones, Daniel Buchan, Tim Nugent, Federico Minneci & Kevin Bryson Previous Contributors Anna Lobley, Sean Ward, Liam J. McGuffin For queries regarding PSIPRED: psipred@cs.ucl.ac.uk. Sednterp.