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Radioimmunoassay - Creative BioMart. Home / Services /Radioimmunoassay Radioimmunoassay Creative BioMart provides Radioimmunoassay (RIA) that uses antibodies to detect and quantitate the amount of antigen (analyte) in a sample. This assay is typically very sensitive and specific. It is possible to detect as low as a few picograms of analyte in the experimental tube when using antibodies of high affinity (Kd = 10-8 - 10-11 M). The basic principle of radioimmunoassay is competitive binding, where a radioactive antigen ("tracer") competes with a non-radioactive antigen for a fixed number of antibody or receptor binding sites. When unlabeled antigen from standards or samples and a fixed amount of tracer (labeled antigen) are allowed to react with a constant and limiting amount of antibody, decreasing amounts of tracer are bound to the antibody as the amount of unlabeled antigen is increased. 125-I labels are usually applied although other isotopes such as C14 and H3 have also been used.

Transporter Screening Assays - Creative BioMart. Transporters and channels control the uptake and efflux of essential nutrients, ions, cellular waste and drugs into and out of cells and organelles. Transporters mediate drug disposition and are major determinants of the pharmacokinetic, safety and efficacy profiles of drugs.

Recent studies have shown that the inhibition or activation of a specific transporter that involves in the absorption, disposition, metabolism, elimination and toxicity of a drug could have significant clinical effects ranging from lowering drug efficacy to compromising safety etc. Transporters are implicated in the pathophysiology of disorders across several therapeutic areas including asthma, cardiovascular disease, diabetes and neuroscience, and therefore, represent attractive targets for drug discovery. Drug transporters are composed of two major super families: ABC (ATP binding cassette) and SLC (solute carrier) transporters.

Technologies for Transporter Drug Discovery. Protein Pathway Profiling - Creative BioMart. Signaling pathways are protein networks involved in multiple cell-development processes like apoptosis, angiogenesis, proliferation, differentiation and so on. Creative BioMart specializes in cell pathways profiling. Our specific antibodies enable to analyze the dysregulation and phosphorylation status of key cell signaling pathways studied in drug discovery and therapeutic development. Main applications: Study of disease mechanisms in biological samples or fixed tissues Identification of pathways involved in drug resistance Early evaluation of drug efficacy (dose response, time course) and mechanism of action in cell based assays Evaluation of combination therapies Fingerprint comparison of biologics and biosimilar activity Assessment of in vitro or animals models As far as you know which pathway you want to profile, we will discuss together to select the key targets to profile.

Please contact us for a quote or for further information. Protein Microarray Service - Creative BioMart. Protein Microarrays can be based on any ligand-binding assay that relies on the formation of product with an immobilized capture molecule and a target molecule present in the solution. These assays can be miniturized and placed on a protein microarray chip. Protein microarrays are now becoming very popular due to their possible future applications in the study of nucleic acid-protein, protein-protein, ligand-receptor, drug-protein target, and enzyme-substrate interactions. Creative BioMart now offers customizable parallel synthesis and microfluidic technologies to create a microarray of thousands of peptides for binding to your protein or antibody sample and a comprehensive protein and/or antibody analysis service utilizing these high density peptide microarrays for high throughput detection, concentration titration, and screening applications.

Our universal microarray platform empowers us to provide: Applications. Metabolism Assays - Creative BioMart. Home / Services /Metabolism Assays Metabolism Assays Metabolism is the set of life-sustaining chemical transformations within the cells. Metabolism reactions allow living cells to grow and reproduce, maintain their structures, and respond to their sress. Metabolism is also refered to nutrition and the availability of nutrients. Bioenergetics describe the metabolic pathways by which the cell ultimately obtains energy. In Vitro Assays Cytochrome P450 inhibition Metabolic stability (microsomes, hepatocytes) Metabolite profiling and identification Multiple species cross-comparisons CYP450 reaction phenotyping Metabolic drug-drug interactions Protein binding (HTS fluorescence HSA, AGP binding), equilibrium HERG channel inhibition Aqueous solubility Fatty Acids Related Assays Straightforward procedure Easy to perform, just add-mix-read Sensitive, accurate, and reliable WIDE assay range Easy to calculate results Fluorometric and/or colorimetric detection Suitable for high-throughput assays.

Mammalian Expression Systems - Creative BioMart. Home / Services / Custom Protein Service /Mammalian Expression Systems Mammalian Expression Systems The main advantage of mammalian cell expression is that the mammalian cells can properly and efficiently recognize the signals for synthesis, processing and secretion of eukaryotic proteins. Therefore expression systems utilizing mammalian cells for recombinant protein manufacture are able to introduce proper protein folding, post-translational modifications, and protein assembly, which are important for complete biological activity. Extending to the pharmaceutical field, almost all biopharmaceutical companies in the world rely on the use of mammalian-cell-based stable cell lines for the manufacture of biologics.

Creative BioMart has decades of experience and expertise in both transient and stable expression based on HEK293 or CHO cell lines. Purification Methods Affinity Chromatography Ion Exchange Chromatography Hydrophobic Interaction Chromatography Size Exclusion Chromatography Reference: Protein Ubiquitinylation Service - Creative BioMart. As a leading biotechnology services supplier, Creative BioMart offers protein ubiquitinylation services. With our expertise as a leader in the field of ubiquitinylation services, Creative BioMart has created a set of new platform technologies. Ubiquitin Screening Service Creative BioMart provides compound screening and profiling against E3 ligase cascades.

Each ubiquitination cascade consists of the three enzyme components (E1 activating enzyme, E2 conjugating enzyme and E3 ligase), plus a biologically-relevant substrate. The incorporation of ubiquitin into the substrate is quantitatively detected by electrochemiluminescence, which allows for the identification of compound inhibitors and activators. Highlights Validated assays for an expanding panel of E3 ligase cascades to screen and profile inhibitors and enhancers. Ubiquitin E3 Ligase Identification Service The service includes the following steps: A high-content, functional microarray is probed with client's substrate. Baculovirus-Insect Cell Expression - Creative BioMart.

The baculovirus-insect cell expression system utilizes recombinant baculoviruses (insect viruses) and their ability to manufacture high yields of biologically active proteins from infected insect cells. The proteins of interest can be easily purified from infected cells or their supernatants using tag and affinity chromatography. At Creative BioMart, our team of experts have developed baculovirus-insect cell expression platform for virus production and recombinant protein manufacture in various scales. The ability of Creative BioMart’s baculovirus-insect cell system to express proteins with complex post-translational modifications (PTMs), combining with high production yield, makes it perfectly suitable for generating mammalian proteins, especially cellular signaling proteins including protein kinases, protein phosphatases and cellular protein substrates, amongst others.

Figure 1. As well as some insect cell line options available at Creative BioMart: Figure 2. Reference: Oxidative Stress Assay - Creative BioMart. Oxidative stress can reflect a disturbance between the systemic production of reactive oxygen species (ROS) and antioxidant defenses against radicals. Oxidative stress is very important and discovered over the past 40 years as an important factor in many forms of pathophysiology generally associated with aging. ROS can react with proteins, nucleic acids, and can cause lipids cell and tissue damage and can be measured using selective or general indicators.

Creative BioMart has a series of systems to do oxidative stress assay. We develop some oxidative biomarkers to perform oxidative stress assay. Lipid peroxidation analysis Lipid peroxidation is a well-demonstrated mechanism of cellular which injury in both plants and animals. Glutathione analysis Glutathione (GSH) is an important antioxidant in animals, fungi and plants. Protein SUMOylation Assay - Creative BioMart. Creative BioMart provides protein SUMOylation assay system for generating SUMOylated proteins in vitro. We provide carboxy-terminal SUMO-1, SUMO-2 or SUMO-3 peptide substrates which are linked covalently to specific lysine residues on the target protein via isopeptide bonds.

Western blot analysis of in vitro SUMOylation of p53 protein by wild-type and mutated isoforms of SUMO-1: The two Western blots were incubated with p53 antibody (1:5000 dilution) and SUMO-1 antibody (1:4000 dilution). The p53 protein is SUMOylated only by the wild-type SUMO-1 protein. Applications Investigation of SUMOylation effects on transcription factor activity Understanding the role of SUMOylation in the regulation of cellular processes Identification of novel proteins as targets for SUMO Please get in contact with Creative BioMart for more information or a detailed discussion. Yeast Expression Systems - Creative BioMart. As a well-defined and economical eukaryotic expression system for both secretory and intracellular expression, yeast protein expression system is perfectly suitable for large-scale manufacture of recombinant proteins.

Creative BioMart has developed yeast expression vectors that are optimized for the efficient production of heterologous proteins. We have also a few strong signal sequences to ensure the highest secretion level. All of below advantages make yeast expression system an ideal host for producing large quantities of properly folded proteins in a short period of time: High yield High productivity Genetics can be easily manipulated Chemically defined media Complex post-translational modifications Stable production strains Durability Ease of scaling up Low protein production cost At Creative BioMart, our expertise in protein expression extends to both Saccharomyces. cerevisiae and Pichia. pastoris expression systems.

YIp YCp YEp Saccharomyces. cerevisiae: Native Proteins - Creative BioMart. More Than 35,000 Natural Products, More coupon waiting for you to take! How to get a coupon? 45-1 Ramsey Road, Shirley, NY 11967, USA Phone: 1-631-559-9269 1-631-448-7888 Fax: 1-631-938-8127 Email: info@creative-biomart.com Europe Phone: 44-203-287-8296 Email: info@creative-biomart.com Native Proteins Background In biochemistry, the native state of a protein is its properly folded and assembled form with operative structure and function. Compared to recombinant proteins generated using protein engineering techniques, native proteins are obtained from proper organisms and are unaltered by outer factors such as heat, chemicals and enzyme reaction, and retain their naturally folded state.

All native proteins provide by Creative BioMart are in stable formulation, retaining their entire activities. Native and Denatured State of Proteins The native state of a protein is defined as the ensemble of compact conformations, forming under folding conditions in vivo or in vitro. How to get a coupon? Europe. Recombinant Proteins - Creative BioMart. Recombinant protein is a manipulated form of protein encoded by recombinant DNA, which has been cloned in a foreign expression system to support the expression of the exogenous gene.

This recombinant DNA construct can be used to manufacture large quantities of useful protein products. The recombinant DNA, usually the cDNA sequence of the target protein, is designed to be under the control of a well-characterized promoter to express the target protein within the chosen host cell to achieve high-level protein expression. Different protein expression systems have different features and applications. Creative BioMart provides multiple sources of recombinant proteins to meet your specific requirements. Recombinant protein CRO services and related products Creative BioMart provides a wide range of off-the-shelf recombinant protein products. We also can custom-produce according to your exact request if your target is not in our catalog. For bulk protein order, please inquire for detail. Protein Glycosylation Labeling Service- Creative BioMart.

As a leading biotechnology services supplier, Creative BioMart offers accurate solutions for characterizing glycan structure and monitoring glycan profiles using mAb-Glyco Chip technology, LC/MS, liquid chromatography systems, and electrophoresis. We provide the following services: Annotate N-linked glycosylation. Annotate O-linked glycosylation. Annotate C-linked glycosylation. Annotate glycationn. Glycan staining or labeling. Lectins can be used to detect and analyze glycoprotein function. Glycoprotein identification. Creative BioMart can advise on the selection of the most appropriate scheme for the experiment. Protein Ubiquitinylation Service. Protein Acetylation Assay.

As a leading biotechnology services supplier, Creative BioMart offers Histone acetylation assay and site identification services. With our expertise as a leader in the field of acetylation, Creative BioMart has created a set of new platform technologies. Creative BioMart has a convenient package of tools that specifically measures global acetylation of histone H3 at tremendously fast speeds and consistency, superior and safer than all other current methods. This is suitable for specifically measuring global histone H3 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

Why choose Creative BioMart? Quick and efficient. Creative BioMart provides all kinds of Protein acetylation assays. Protein Methylation Assay. Protein Kinase Assay. Protein Sequence Analysis and Function Prediction. Protein Labeling. Luciferase Reporter Assay. Protein Characterization. Biosafety Testing-Creative BioMart. Protein Phylogenetic Analysis.

Protein Sequence Analysis and Function Prediction. Protein Kinase Assay. ATPase/GTPase Assay. High-Throughput Screening. Cytokine and Receptor Analysis. Protein Phosphorylation. Calcium-Binding Proteins And Related Molecules. Jak/Stat Signaling Proteins. Cytoskeleton / Ecm Proteins. Protein Kinase. Recombinant Human MAPKAPK3, GST tagged MAPKAPK3-146H. Recombinant Human BMPR2, His tagged BMPR2-134H. Recombinant Human ACY1, His tagged ACY1-242H. Recombinant Human SPG21, GST tagged SPG21-872H. Recombinant Human PRLR, His tagged PRLR-177H. Membrane Protein Services. Membrane-Based Yeast Two-Hybrid System -Creative BioMart. Mammalian Two-Hybrid System -Creative BioMart. Epitope Mapping. Enzyme Activity Assay. Chromatography. Crystallography, Transport Protein - Creative Biomart. Prl Protein, prolactin.

Enzyme Inhibitors, Pharmaceutical Enzymes, Diagnostic Enzymes. Native Proteins | Native Proteins Database - Creative Biomart. Novel Approach for Identifying Protein Interaction Was Found | Creative Biomart Blog. Do you regard GMO-labels as negative warnings? | Creative Biomart Blog. Dog Sniffs Cancer, Do You Believe It? | Creative Biomart Blog. Month-end Check: Biotech study topics in Nature-July (Part TWO) | Creative Biomart Blog. Month-end Check: Biotech study topics in Nature-July (Part One) | Creative Biomart Blog. Crystallography, Transport Protein - Creative Biomart. Chromatography. Fluorescent Proteins - Creative Biomart. Dialysis. Therapeutic Proteins Stable Cell Line-Creative Biomart. Creative BioMart @ TheHubEdu.com. Blueprinting Proteins, Once Difficult Now Easier | News | CamTechNet | The Cambridge Technology & Jobs Community. Diagnostic Antigen - Creative Biomart. Lysates,Tissue and Cell Lysates.

Protein reference standard. GMP Proteins. Recombinant Proteins. Protease Assay Service. Ion Channel Screening Assays. Epitope Mapping. Oxidative Stress Assay. Luciferase Reporter Assay. Codon Optimization. Epigenetics-Based Assay Development. Protein Microarray Service. Electron Transfer Quenching Platform. cAMP Accumulation Assay. Protein Pathway Profiling. Transporter Screen Assay. Radioimmunoassay. Cytokine and Receptor Analysis. Biomarker Service. Transcription Factor Assay- Creative BioMart.

Biosimilar Comparability Studies. Biosafety Testing-Creative BioMart. Downstream Processing Development. Pharmaceutical Stability Analysis. GLP-Compliant Bioprocess Services. Biopharmaceutical Process and Product Related Impurities Analysis. cGMP cell-based potency assays. Product Release tTesting of Pharmaceuticals. Quality Control-Creative BioMart. Protein Expression and Purification Services - Creative BioMart. Protein Expression and Purification Services - Creative BioMart. Protein Interaction Service - Creative BioMart.

Protein Labeling - Creative BioMart. Mammalian Expression Systems (CHO / 293) - Creative BioMart. Yeast Expression Systems (P pastoris / S cerevisiae) - Creative BioMart. Bacterial Expression Systems (E. coli / Bacillus) - Creative BioMart. Protein Expression and Purification Services - Creative BioMart. Protein Expression Microarray - Creative BioMart.