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Protein Phosphorylation. Protein Phosphorylation Background Protein phosphorylation is an important post-translational modification on proteins, which is responsible for many biological functions.

Protein Phosphorylation

The neutral hydroxyl groups on the protein (serine, threonine and tyrosine) can be changed to a charged phosphate group by the protein phosphorylation process. As a reverse reaction, the phosphoprotein can also be dephosphorylated by protein phosphatases. Protein phosphorylation plays a key role in many cellular functions including the nervous and immune system and regulation of transcription.

Many human diseases, such as cancer, inflammation and diabetes, occur due to the abnormal activity of protein phosphorylation. Calcium-Binding Proteins And Related Molecules. Calcium Binding Proteins And Related Molecules Background Calcium binding proteins are a class of proteins that bind free calcium ions within the cell.

Calcium-Binding Proteins And Related Molecules

One particular type of highly characterized calcium binding proteins is the EF-hand calcium binding proteins. There are more than 250 EF-hand proteins, and they all share a common structural motif, a Ca2+-binding loop between two a-helices oriented perpendicular to each other. The term comes from a description of the C-terminal E-helix-loop-F-helix Ca2+-binding site in parvalbumin. Members of the EFhand family of calcium binding proteins include calbindin (CB), calretinin (CR), parvalbumin (PV), calcineurin, aequorin, and calmodulin. Jak/Stat Signaling Proteins. Jak Stat Signaling Proteins Background Cytokines play a pivotal role in regulating a variety of immune responses mainly by downstream gene activation or repression.

Jak/Stat Signaling Proteins

To transduce extracellular signals into intracellular responses, many cytokines utilize a common pathway - the Janus kinase (Jak)-signal transducer and activator of transcription (STAT) signaling pathway. Cytoskeleton / Ecm Proteins. Cytoskeleton Ecm Proteins Background The Cytoskeleton The cytoskeleton is the name of a filamentous network spanning the cytosol.

Cytoskeleton / Ecm Proteins

The initial understanding of the cytoskeleton was o f a scaffolding structure that helps the cell resist external pressures and maintain its shape. This idea was broadened as more of the cytoskeleton’s function came into light. Protein Kinase. Protein Kinase Background Kinases transfer the γ-phosphate of ATP to other biological molecules, serving as chemical messengers that make the tight regulation of cell-signaling pathways possible.

Protein Kinase

For example, non-receptor tyrosine kinases are found in the cytoplasm (not membrane-bound) and transfer the γ-phosphate of ATP to tyrosine residues of other proteins, often turning these proteins “on” or “off” in the context of their respective signaling pathway. This notion of “on” or “off” can be useful to holistically conceptualize these pathways but is a gross oversimplification; in reality, many of these enzymes are multi-domain proteins that can exist in multiple conformational states, participate in multiple protein-protein interactions, and experience a gradient of variable activity levels depending on these states. The active conformation of kinases is highly conserved across almost all non-metabolic, Eukaryotic Protein Kinases. Recombinant Human MAPKAPK3, GST tagged MAPKAPK3-146H.

Recombinant Human BMPR2, His tagged BMPR2-134H. Recombinant Human ACY1, His tagged ACY1-242H. Recombinant Human SPG21, GST tagged SPG21-872H. Recombinant Human PRLR, His tagged PRLR-177H. Membrane Protein Services. Creative BioMart is a biotechnology company committed to addressing the unmet needs of drug discovery for the treatment and cure of human diseases that involve membrane proteins.

Membrane Protein Services

Membrane proteins such as receptors and ion channels are key regulators of cellular function. Membrane proteins account for up to two thirds of known drugable targets, highlighting their critical pharmaceutical importance. One of the largest, most versatile group of membrane receptors, the G protein-coupled receptors (GPCRs), is also the most pharmaceutically important, accounting for over 50% of all human drug targets and acting as therapeutic targets for a wide range of disease conditions including cancer, cardiovascular, metabolic, CNS and inflammatory diseases. Membrane-Based Yeast Two-Hybrid System -Creative BioMart. The classical yeast two-hybrid system and its modifications have been successfully used over the past decade to investigate interactions between most classes of proteins expressed in a given cell or tissue.

Membrane-Based Yeast Two-Hybrid System -Creative BioMart

Mammalian Two-Hybrid System -Creative BioMart. Creative BioMart Mammalian Two-Hybrid Assay allows rapid and convenient analysis of protein-protein interactions in transfected mammalian cells.

Mammalian Two-Hybrid System -Creative BioMart

This assay uses a SEAP secreted reporter to measure the interactions between the two proteins of interest, eliminating the need for cell lysis. Because this two-hybrid assay is performed in mammalian cells, proteins encoded by mammalian cDNAs are more likely to be in their native conformation; therefore, post-translational modifications and experimental results are more likely to represent biologically significant interactions. Features. Epitope Mapping. Epitope mapping has become more and more vital in both vaccine and antibody drug development.

Epitope Mapping

Knowledge on epitope of an antibody will largely facilitate your drug design and patent application. Based on our advanced peptide library platform, Creative BioMart provides a comprehensive series of Epitope Mapping Services to fulfill customers' demand. Customer should submit the antigen sequence, antigen sample, target antibody sample, and control antibody sample (optional) for the services. Our customer service representatives are available 24 hours a day. Enzyme Activity Assay. The vast majority of catalyzing biological processes is performed by enzymes which provide a wide variety of functions inside living organisms.

Enzyme Activity Assay

They are indispensable for metabolic processes, cellular signaling and regulation and can generate movement and active transport of molecules. Enzymatic reactions specifically convert substrate molecules into chemically modified products. These modifications include proteolytic or glycolytic degradation, chemical modifications such as phosphorylation, acylation, glycosylation or oxidative and reductive processes. Dysregulated enzyme activity can lead to disease states. As a result enzymes are considered to be attractive targets for pharmacological intervention in drug therapy.

Chromatography. Crystallography, Transport Protein - Creative Biomart. Prl Protein, prolactin. Enzyme Inhibitors, Pharmaceutical Enzymes, Diagnostic Enzymes. Creative BioMart offers a comprehensive collection of enzymes and enzyme inhibitors covering all the core biological/pharmacological research areas. Enzymes, proteins that catalyze chemical reactions without being used in the reaction, are fundamental to all biological processes. Typically enzymes bind a substrate in a well confined binding pocket which stabilizes the intermediate state that the substrate has to assume during its conversion into the product of the enzymatic reaction.

They are useful in various ways in different areas, such as diagnostics, industry and pharmaceutics, etc. An enzyme inhibitor is a molecule, which binds to enzyme and subsequently stops a substrate from entering the active site of enzyme and/or hinders the enzyme from catalysis. Since blocking an enzyme activity can have profound effects on the downstream cellular events sometimes killing pathogens or regulating metabolic balance, many drugs are enzyme inhibitors. Native Proteins Database - Creative Biomart. Novel Approach for Identifying Protein Interaction Was Found. Do you regard GMO-labels as negative warnings? In the last two posts we have talked about bioengineering. I still want to lay my today’s topic on GMO. Dog Sniffs Cancer, Do You Believe It? Dogs can help sniff cancer, though sounds incredible, this research has made a huge steep forward these years.

Today, the “Cancer sniffing dogs project” has been approved by British NHS, entering into the testing phase, in order to further validate this uncanny ability of dogs. Actually, the ability of dogs to smell cancer is well documented. A 1989 report marked the first medical record of this ability. And the following studies have quantified how skilled dogs are at detecting different cancers including lung, breast and bladder cancers. Month-end Check: Biotech study topics in Nature-July (Part TWO) Month-end Check: Biotech study topics in Nature-July (Part One) At the end of this month, I’d like to make a summary about the biotechnology studies published in Nature, aiming to provide a clear view of the monthly studies’ topics. And the studies were listed chronologically.

Moncef Slaoui This is a review. Based on an event that GlaxoSmithKline (GSK) surprised the industry with its decision to trade its oncology business for Novartis’ vaccine business last year. This article mainly discussed 7 questions which include: “Why did you trade your oncology business for Novartis’ vaccine business?” , “But whereas the oncology market is booming, the vaccine market has been flat for the past few years. Crystallography, Transport Protein - Creative Biomart. Chromatography. Fluorescent Proteins - Creative Biomart. Dialysis. Dialysis is an easy and efficient technique to separate biomolecules according to their molecular weight and for the purification of proteins, DNA, RNA and oligonucleotide.

Furthermore dialysis is also useful for small bioactive compounds release or binding studies. The MWCO (Molecular Weight Cut Off) is the cut frontier, which size of molecules can pass through the membrane. The MWCO is always stated in Dalton (D or Da) and describes also the typical pore size of the membrane. Therapeutic Proteins Stable Cell Line-Creative Biomart. Creative BioMart @ Recently, a new study has brought hope to the blueprinting of a certain proteins, which is a big step forward in the field of drug discovery and development. Named in meso in situ serial crystallography (IMISX), this new method makes the job of blueprinting certain proteins relatively faster, cheaper and easier. Actually, over half of the drugs on the market cell membrane proteins, which are important for the everyday functioning of complex cellular processes.

However, the big problem we were facing is that the production of large membrane protein crystals, which are used to determine the precise 3-D structural blueprints. And previously, scientists need to harvest protein crystals and cool them at inhospitable temperature. While those acts were mostly damaging, inefficient and more prone to errors. Martin Caffrey, the senior author of the research commented: “This discovery is really an exciting thing. Blueprinting Proteins, Once Difficult Now Easier. Recently, a new study has brought hope to the blueprinting of a certain proteins, which is a big step forward in the field of drug discovery and development.

Diagnostic Antigen - Creative Biomart. Lysates,Tissue and Cell Lysates. Protein reference standard. GMP Proteins. Recombinant Proteins. Protease Assay Service. Approximately 500 putative proteases have been identified in human genome. Through controlling complex proteolytic activities, proteases participate in a wide range of physiological processes. Ion Channel Screening Assays. Epitope Mapping. Oxidative Stress Assay. Oxidative stress can reflect a disturbance between the systemic production of reactive oxygen species (ROS) and antioxidant defenses against radicals.

Oxidative stress is very important and discovered over the past 40 years as an important factor in many forms of pathophysiology generally associated with aging. ROS can react with proteins, nucleic acids, and can cause lipids cell and tissue damage and can be measured using selective or general indicators. Luciferase Reporter Assay.

Codon Optimization. Epigenetics-Based Assay Development. As one of the booming fields, epigenetics bridges genotype to phenotype and provides in-depth understanding of aging, human development, the origins of cancer, metabolic diseases, heart disease, mental illness, as well as several other diseases. Protein Microarray Service. Protein Microarrays can be based on any ligand-binding assay that relies on the formation of product with an immobilized capture molecule and a target molecule present in the solution. Electron Transfer Quenching Platform. Quenching refers to any process which decreases the fluorescence intensity of a given substance.

It refers to reduction of fluorescence intensity by another molecule, one that causes some of the excited fluorophore to return to their ground states without emitting photons. Quenching is used in optode sensors; for instance the quenching effect of oxygen on certain ruthenium complexes allows the measurement of oxygen saturation in solution.

cAMP Accumulation Assay. Protein Pathway Profiling. Transporter Screen Assay. Radioimmunoassay. Cytokine and Receptor Analysis. Biomarker Service. Transcription Factor Assay- Creative BioMart. Biosimilar Comparability Studies. Biosafety Testing-Creative BioMart. Downstream Processing Development. Pharmaceutical Stability Analysis. GLP-Compliant Bioprocess Services. Biopharmaceutical Process and Product Related Impurities Analysis. cGMP cell-based potency assays. Product Release tTesting of Pharmaceuticals. Quality Control-Creative BioMart. Protein Expression and Purification Services - Creative BioMart. Protein Expression and Purification Services - Creative BioMart. Protein Interaction Service - Creative BioMart.

Protein Labeling - Creative BioMart. Mammalian Expression Systems (CHO / 293) - Creative BioMart. Yeast Expression Systems (P pastoris / S cerevisiae) - Creative BioMart. Bacterial Expression Systems (E. coli / Bacillus) - Creative BioMart. Protein Expression and Purification Services - Creative BioMart. Protein Expression Microarray - Creative BioMart.