UCB Barker-Koshland storeroom. Our Markup Las year, we lowered our markup from 14% to 9%. We had increased sales so much that we were able to run on a smaller percentage of the take. We knew at the time that this wouldn't last. The 9% rate meant that we were paying down the vast surplus that had built up over the previous year.
So it came as no surprise when our accountant in BDS told us we had to raise it. So we raised it. That's right: Our work saving you money has been so effective that our more permanent sale price is just barely above our "holy crap we should get rid of this money" price. We are thrilled to remain the best way to buy most of the things that make science go. 16 GB Flash Drives are now $12.99! That's not very much money! (Updated 8/12/13) Rainin Pipet-Lite XLS--extremely cheap. Rainin has recently discontinued its Pipet-Lite series, and replaced it with Pipet-Lite XLS. They're still giving us an awesome deal, though.
As always, our prices include tax. New Lower Prices! Save on Shipping (Updated 10/1/10) Dry Ice. UCB DNA Sequencing Facility. UCB Electron Microscope Lab. Tuesday 04-08-14 04:20:43 PM The Robert D. Ogg Electron Microscope Laboratory (EML) is located in 26 Giannini Hall in the North basement. The EML is an instruction and research unit of the College of Letters and Sciences, providing EM services to the Berkeley campus and the academic community at large. The EML provides two basic types of service: Education and training Equipment for trained users All are available to anyone with an interest in electron microscopy: graduate and undergraduate students, post-doctoral researchers, faculty, staff and non-UC users. EDUCATION AND TRAINING The EML provides basic to advanced training in all aspects of transmission electron microscopy (TEM) and scanning electron microscopy (SEM), from specimen preparation to image processing. Ciliaproteome. Cold Spring Harbor Protocols. Tabin lab RNA Whole Mount In Situ Hybridization.
RNA Whole Mount In Situ Hybridization Core protocol for both mouse and chick embryos Cepko/Tabin lab Embryo preparation Fix embryos in fresh 4% paraformaldehyde (or a thawed frozen aliquot) in PBS at 4° C overnight. Dissect embryos (as needed) into PBT, and wash once in PBT for 5 minutes with gentle rocking. Dehydrate embryos into methanol using a graded methanol/PBT series (25%, 50%, 75%, 100% methanol). Allow embryos to rock gently at room temperature for 5 minutes in each wash solution. Day 1: Prehybridization and Hybridization Rehydrate samples in a 75%, 50%, 25% methanol/PBT series.
Day 2: Post-hybridization washes, blocking, and antibody incubation Prewarm solution I to 70°C. Day 3: Post-antibody washes Wash 3 times for 5 minutes each with TBST at room temperature. Day 4: Color development Wash 3 times in NTMT for at least 10 minutes each at room temperature. Solutions All solutions should be made with high-quality reagent grade water that is RNase-free. 10X PBS (1 liter): 80 g NaCl. Protocols Directory. AG. Gene Ontology. DBGET database. Entrez cross database.
SCOP: Structural Classification of Proteins. Authors. Alexey G. Murzin, John-Marc Chandonia, Antonina Andreeva, Dave Howorth, Loredana Lo Conte, Bartlett G. Ailey, Steven E. Brenner, Tim J. P. Hubbard, and Cyrus Chothia. scop@mrc-lmb.cam.ac.uk Reference: Murzin A. The prototype of a new Structural Classification of Proteins 2 (SCOP2) database is now available at Access methods Enter SCOP at the top of the hierarchy Keyword search of SCOP entries SCOP parseable files (MRC site) All SCOP releases and reclassified entry history (MRC site) pre-SCOP - preview of the next release SCOP domain sequences and pdb-style coordinate files (ASTRAL) Hidden Markov Model library for SCOP superfamilies (SUPERFAMILY) Structural alignments for proteins with non-trivial relationships (SISYPHUS) Online resources of potential interest to SCOP users SCOPmirrors around the world may speed your access.
News SCOP has been updated to include many PDB entries released before 23 February 2009. Synopsis. ModBase Comparative Protein Structure Models. Model Details Example Database ID: Q12321 This page makes all models and model details for one sequence available. The current model is shown prominently. Additional models are accessible through their thumbnails. Use the Perform action pull-down menu for Coordinates Alignments Various ModBase representations links to UCSF Chimera Overview Example Dengue Virus Models for several sequences are displayed on the Sequence Overview page. A coverage sketch illustrates sequence section(s) covered by a model The sketch also links to the Model Details page.
Users of ModBase are requested to cite this article in their publications: MODBASE, a database of annotated comparative protein structure models and associated resources.Ursula Pieper, Ben M. EMBL EBI Macromolecular Structure Database Group. SUPERFAMILY database of structural and functional protein annota. PANTHER - Classifications of Genes and Proteins. PRINTS. SIB ExPASy SWISS-2DPAGE. SIB ExPASy World-2DPAGE Repository. UniProt UniPathway Metabolic data. Gene3D. TIGRFAMS. ProDom. Pfam. UniProt. OrthoMCL. Chlamydomonas Flagellar Proteome. Ciliome. Geneious. PAML - Phylogenetic Analysis by Maximum Likelihood. Ziheng Yang Table of contents Introduction PAML is a package of programs for phylogenetic analyses of DNA or protein sequences using maximum likelihood.
It is maintained and distributed for academic use free of charge by Ziheng Yang. ANSI C source codes are distributed for UNIX/Linux/Mac OSX, and executables are provided for MS Windows. PAML is not good for tree making. It may be used to estimate parameters and test hypotheses to study the evolutionary process, when you have reconstructed trees using other programs such as PAUP*, PHYLIP, MOLPHY, PhyML, RaxML, etc. This document is about downloading and compiling PAML and getting started. Downloading and Setting up PAML PAML-X: A GUI for PAML A graphical user interface, called PAML-X, has been written by Bo Xu of Institute of Zoology, Chinese Academy of Sciences in Beijing.
The following is written for the naive user. PAML for Windows 9x/NT/2000/XP/Vista/7 Setting up a folder of local programs and changing the search path. Tar xzf paml4.7a.tgz. VoodooPad. Tabs Open pages in multiple tabs, all in the same window. Support for big documents Stuff gigabytes of data inside a VoodooPad document, it won't mind. Embed images, PDFs, and other types of files. VoodooPad can embed files such as images, PDFs, mp3s, and most other types of files as well. Print to VoodooPad PDF Service Print from any application as a PDF, and stick it in VoodooPad for safe keeping.
Script plugins Script Plugins are an easy way to extend the functionality of VoodooPad. Palettes Find your backlinks, pages list, tags, recently viewed pages, and document info quickly and easily. Text styles Create, manage, and apply custom text styles available from a floating palette or the menubar - all within VoodooPad. Merge and split Merge two VoodooPad documents together using the import menu option, or split pages from a document into a new file to slim things down a bit. Events Item meta Web server Full screen editing Web export Intelligently export your pages as HTML documents.
Tags Sketch Encryption. The Omni Group. ImageJ. TextMate Manual. Generic Model Organism Database. OBO-Edit. MUSCLE. MrBayes. O'Reilly Digital Media Center. UNIX and Linux Index Page. Home Software that I have written. Linux Linux Book-marks Replacing Win95? Video Modes My perl program that generates modelines for XFree86 Tutorials Useful information mostly written by me, ( with the exception of the bash manpage ) The Font HOWTO. Linux Software Reviews- Word Processors Graphics tools (User Friendly)Text Editors GUI Mailtools File managers and FTP clients Project Independence An ease of use oriented linux project that I am working on.
Quotes From Linux Hardware Shops Medium cost IDE boxes Want linux pre-installed, but can't afford a machine from VaResearch ? SCSI Dream Machines Want a SCSI dream machine with linux pre-installed ? LDP products page Has a very good list of linux hardware vendors. My Vim page. Subversion. FOLDOC Computing Dictionary. Bash Reference Manual: This text is a brief description of the features that are present in the Bash shell (version 4.3, 2 February 2014). The Bash home page is This is Edition 4.3, last updated 2 February 2014, of The GNU Bash Reference Manual, for Bash, Version 4.3. Bash contains features that appear in other popular shells, and some features that only appear in Bash. Some of the shells that Bash has borrowed concepts from are the Bourne Shell (`sh'), the Korn Shell (`ksh'), and the C-shell (`csh' and its successor, `tcsh').
This manual is meant as a brief introduction to features found in Bash. 1.1 What is Bash? Bash is the shell, or command language interpreter, for the GNU operating system. Bash is largely compatible with sh and incorporates useful features from the Korn shell ksh and the C shell csh. While the GNU operating system provides other shells, including a version of csh, Bash is the default shell. 1.2 What is a shell? Blank A space or tab character. builtin field \a. Bash Guide for Beginners. MacScripter Applescript and Scripting Resources. Technology Review: The Authority on the Future of Technology.